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Title:
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Experimental Verification of Dielectric Models with a Capacitive Wheatstone Bridge Biosensor for Living Cells: E. coli
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Author:
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Zarrinkhat, Faezeh; Jofre-Roca, Lluis; Jofre, Marc; Rius, Juan M.; Romeu, Jordi
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Other authors:
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[Zarrinkhat F, Jofre-Roca L, Rius JM, Romeu J] CommSensLab, Universitat Politecnica de Catalunya (UPC), Barcelona, Spain. [Jofre M] CommSensLab, Universitat Politecnica de Catalunya (UPC), Barcelona, Spain. Hospital General de Granollers, Granollers, Spain; Hospital General de Granollers |
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Abstract:
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Dielectric spectroscopy; E. coli bacteria; Maxwell–Garnet model |
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Abstract:
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Espectroscopia dieléctrica; Bacterias E. coli; Modelo Maxwell-Garnet; |
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Abstract:
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Espectroscòpia dielèctrica; Bacteris E. coli; Model Maxwell-Garnet |
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Abstract:
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Detection of bioparticles is of great importance in electrophoresis, identification of biomass sources, food and water safety, and other areas. It requires a proper model to describe bioparticles' electromagnetic characteristics. A numerical study of Escherichia coli bacteria during their functional activity was carried out by using two different geometrical models for the cells that considered the bacteria as layered ellipsoids and layered spheres. It was concluded that during cell duplication, the change in the dielectric permittivity of the cell is high enough to be measured at radio frequencies of the order of 50 kHz. An experimental setup based on the capacitive Wheatstone bridge was designed to measure relative changes in permittivity during cell division. In this way, the theoretical model was validated by measuring the dielectric permittivity changes in a cell culture of Escherichia coli ATTC 8739 from WDCM 00012 Vitroids. The spheroidal model was confirmed to be more accurate. |
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Subject(s):
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-Espectroscòpia d'electrons
-Escheríchia coli
-Models geomètrics
-ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Investigative Techniques::Chemistry Techniques, Analytical::Spectrum Analysis::Dielectric Spectroscopy
-ORGANISMS::Bacteria::Gram-Negative Bacteria::Gram-Negative Facultatively Anaerobic Rods::Enterobacteriaceae::Escherichia::Escherichia coli
-ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Investigative Techniques::Chemistry Techniques, Analytical::Electrophoresis
-TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::técnicas de investigación::técnicas de química analítica::análisis espectral::espectroscopia dieléctrica
-ORGANISMOS::Bacteria::bacterias gramnegativas::bacilos gramnegativos anaerobios facultativos::Enterobacteriaceae::Escherichia::Escherichia coli
-TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::técnicas de investigación::técnicas de investigación::técnicas electroquímicas::electroforesis |
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Rights:
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Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
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Document type:
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Article
Article - Published version |
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Published by:
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MDPI
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