dc.contributor
Institut Català de la Salut
dc.contributor
[Duarri A] Program for Clinical Translation of Regenerative Medicine in Catalonia–P-CMR[C], Bellvitge Biomedical Research Institute (IDIBELL), Hospitalet de Llobregat, 08908 Barcelona, Spain. National Stem Cell Bank-Barcelona Node, Biomolecular and Bioinformatics Resources Platform PRB2, ISCIII, IDIBELL, Hospitalet de Llobregat, 08908 Barcelona, Spain. Grup de Recerca en Oftalmologia, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. [Rodríguez-Bocanegra E, Biarnés M, García M, Ferraro LL] Barcelona Macula Foundation: Research for Vision, 08022 Barcelona, Spain. Institut de la Màcula, Centro Médico Teknon, 08022 Barcelona, Spain. [Martínez-Navarrete G] Networking Research Center of Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28029 Madrid, Spain. Institute of Bioengineering, Universidad Miguel Hernández, 03202 Alicante, Spain
dc.contributor
Vall d'Hebron Barcelona Hospital Campus
dc.contributor.author
Duarri Piqué, Anna
dc.contributor.author
Rodríguez-Bocanegra, Eduardo
dc.contributor.author
Martínez-Navarrete, Gema
dc.contributor.author
García, Miriam
dc.contributor.author
Ferraro, Lucía Lee
dc.contributor.author
Biarnés, Marc
dc.date.accessioned
2025-10-24T08:51:50Z
dc.date.available
2025-10-24T08:51:50Z
dc.date.issued
2022-05-02T12:56:25Z
dc.date.issued
2022-05-02T12:56:25Z
dc.identifier
Duarri A, Rodríguez-Bocanegra E, Martínez-Navarrete G, Biarnés M, García M, Ferraro LL, et al. Transplantation of Human Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium in a Swine Model of Geographic Atrophy. Int J Mol Sci. 2021 Oct;22(19):10497.
dc.identifier
https://hdl.handle.net/11351/7441
dc.identifier
10.3390/ijms221910497
dc.identifier
000756327600001
dc.identifier.uri
http://hdl.handle.net/11351/7441
dc.description.abstract
Modelo animal; Medicina regenerativa; Retina
dc.description.abstract
Model animal; Medicina regenerativa; Retina
dc.description.abstract
Animal model; Regenerative medicine; Retina
dc.description.abstract
Background: The aim of this study was to test the feasibility and safety of subretinal transplantation of human induced pluripotent stem cell (hiPSC)-derived retinal pigment epithelium (RPE) cells into the healthy margins and within areas of degenerative retina in a swine model of geographic atrophy (GA). Methods: Well-delimited selective outer retinal damage was induced by subretinal injection of NaIO3 into one eye in minipigs (n = 10). Thirty days later, a suspension of hiPSC-derived RPE cells expressing green fluorescent protein was injected into the subretinal space, into the healthy margins, and within areas of degenerative retina. In vivo follow-up was performed by multimodal imaging. Post-mortem retinas were analyzed by immunohistochemistry and histology. Results: In vitro differentiated hiPSC-RPE cells showed a typical epithelial morphology, expressed RPE-related genes, and had phagocytic ability. Engrafted hiPSC-RPE cells were detected in 60% of the eyes, forming mature epithelium in healthy retina extending towards the border of the atrophy. Histological analysis revealed RPE interaction with host photoreceptors in the healthy retina. Engrafted cells in the atrophic zone were found in a patchy distribution but failed to form an epithelial-like layer. Conclusions: These results might support the use of hiPSC-RPE cells to treat atrophic GA by providing a housekeeping function to aid the overwhelmed remnant RPE, which might improve its survival and therefore slow down the progression of GA.
dc.description.abstract
This work was supported by Spanish Ministry of Science and Innovation (MICINN, RTI2018-095377-B-100), Instituto de Salud Carlos III ISCIII/FEDER (PRB2 PT13/0001/0041; TerCel RD16/0011/0024 and Oftared-RETICS RD16/0008), ERA-NET EuroNanoMed III/ISCIII (AC19/00080), the Catalan Government, AGAUR (2017-SGR-899), CERCA Programme/Generalitat de Catalunya, and by Fundació Carmen i Mª José Godó (Fundació CMJ Godó 2017).
dc.format
application/pdf
dc.format
application/pdf
dc.relation
International Journal of Molecular Sciences;22(19)
dc.relation
https://doi.org/10.3390/ijms221910497
dc.relation
info:eu-repo/grantAgreement/ES/PE2017-2020/AC19%2F00080
dc.rights
Attribution 4.0 International
dc.rights
http://creativecommons.org/licenses/by/4.0/
dc.rights
info:eu-repo/semantics/openAccess
dc.subject
Retina - Malalties - Trasplantació
dc.subject
Cèl·lules mare adultes
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Degeneració macular - Cirurgia
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ANATOMY::Sense Organs::Eye::Pigment Epithelium of Eye::Retinal Pigment Epithelium
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Other subheadings::Other subheadings::Other subheadings::Other subheadings::/transplantation
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ANATOMY::Cells::Stem Cells::Adult Stem Cells::Induced Pluripotent Stem Cells
dc.subject
DISEASES::Eye Diseases::Retinal Diseases::Retinal Degeneration::Macular Degeneration::Geographic Atrophy
dc.subject
ANATOMÍA::órganos de los sentidos::ojo::epitelio pigmentado ocular::epitelio pigmentado de la retina
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Otros calificadores::Otros calificadores::Otros calificadores::Otros calificadores::/trasplante
dc.subject
ANATOMÍA::células::células madre::células madre adultas::células madre pluripotentes inducidas
dc.subject
ENFERMEDADES::oftalmopatías::enfermedades de la retina::degeneración retiniana::degeneración macular::atrofia geográfica
dc.title
Transplantation of Human Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium in a Swine Model of Geographic Atrophy
dc.type
info:eu-repo/semantics/article
dc.type
info:eu-repo/semantics/publishedVersion
