dc.contributor
Institut Català de la Salut
dc.contributor
[Seth-Smith HMB] Clinical Bacteriology & Mycology, University Hospital Basel, University of Basel, Switzerland. [Bénard A] Wellcome Trust Sanger Institute, Cambridge, UK. Grup de Recerca en Sistemes Sanitaris, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. [Bruisten SM] Department of Infectious Diseases, GGD Public Health Service of Amsterdam, Amsterdam, The Netherlands. Amsterdam UMC, University of Amsterdam, Amsterdam Institute for Infection and Immunity (AII), Location Academic Medical Centre, Amsterdam, The Netherlands. [Versteeg B] Department of Infectious Diseases, GGD Public Health Service of Amsterdam, Amsterdam, The Netherlands. Clinical Research Department, London School of Hygiene and Tropical Medicine, London, UK. [Herrmann B] Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden. [Kok J] Marie Bashir Institute for Infectious Diseases and Biosecurity & Westmead Clinical School, University of Sydney, Sydney, New South Wales, Australia. Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Westmead, New South Wales, Australia
dc.contributor
Vall d'Hebron Barcelona Hospital Campus
dc.contributor.author
Seth-Smith, Helena M. B.
dc.contributor.author
Bénard, Angèle Helene Marie
dc.contributor.author
Bruisten, Sylvia M.
dc.contributor.author
Versteeg, Bart
dc.contributor.author
Herrmann, Björn
dc.contributor.author
Kok, Jen
dc.date.accessioned
2025-10-24T08:55:08Z
dc.date.available
2025-10-24T08:55:08Z
dc.date.issued
2022-04-06T10:15:12Z
dc.date.issued
2022-04-06T10:15:12Z
dc.date.issued
2021-06-29
dc.identifier
Seth-Smith HMB, Bénard A, Bruisten SM, Versteeg B, Herrmann B, Kok J, et al. Ongoing evolution of Chlamydia trachomatis lymphogranuloma venereum: exploring the genomic diversity of circulating strains. Microb Genomics. 2021 Jun 29;7(6):000599.
dc.identifier
https://hdl.handle.net/11351/7312
dc.identifier
10.1099/mgen.0.000599
dc.identifier.uri
http://hdl.handle.net/11351/7312
dc.description.abstract
Epidemiología molecular; Presión selectiva; Infecciones de transmisión sexual
dc.description.abstract
Molecular epidemiology; Selective pressure; Sexually transmitted infections
dc.description.abstract
Epidemiologia molecular; Pressió selectiva; Infeccions de transmissió sexual
dc.description.abstract
Lymphogranuloma venereum (LGV), the invasive infection of the sexually transmissible infection (STI) Chlamydia trachomatis , is caused by strains from the LGV biovar, most commonly represented by ompA-genotypes L2b and L2. We investigated the diversity in LGV samples across an international collection over seven years using typing and genome sequencing. LGV-positive samples (n=321) from eight countries collected between 2011 and 2017 (Spain n=97, Netherlands n=67, Switzerland n=64, Australia n=53, Sweden n=37, Hungary n=31, Czechia n=30, Slovenia n=10) were genotyped for pmpH and ompA variants. All were found to contain the 9 bp insertion in the pmpH gene, previously associated with ompA-genotype L2b. However, analysis of the ompA gene shows ompA-genotype L2b (n=83), ompA-genotype L2 (n=180) and several variants of these (n=52; 12 variant types), as well as other/mixed ompA-genotypes (n=6). To elucidate the genomic diversity, whole genome sequencing (WGS) was performed from selected samples using SureSelect target enrichment, resulting in 42 genomes, covering a diversity of ompA-genotypes and representing most of the countries sampled. A phylogeny of these data clearly shows that these ompA-genotypes derive from an ompA-genotype L2b ancestor, carrying up to eight SNPs per isolate. SNPs within ompA are overrepresented among genomic changes in these samples, each of which results in an amino acid change in the variable domains of OmpA (major outer membrane protein, MOMP). A reversion to ompA-genotype L2 with the L2b genomic backbone is commonly seen. The wide diversity of ompA-genotypes found in these recent LGV samples indicates that this gene is under immunological selection. Our results suggest that the ompA-genotype L2b genomic backbone is the dominant strain circulating and evolving particularly in men who have sex with men (MSM) populations.
dc.description.abstract
J.C.G. was supported by the Instituto de Salud Carlos III (Plan Estatal de I+D+ i 2013–2016), Grant PI16-01242.
dc.format
application/pdf
dc.publisher
Microbiology Society
dc.relation
Microbial Genomics;7(6)
dc.relation
https://doi.org/10.1099/mgen.0.000599
dc.rights
Attribution-NonCommercial 4.0 International
dc.rights
http://creativecommons.org/licenses/by-nc/4.0/
dc.rights
info:eu-repo/semantics/openAccess
dc.subject
Infeccions per clamídia - Aspectes genètics
dc.subject
Malalties de transmissió sexual - Epidemiologia
dc.subject
ORGANISMS::Bacteria::Gram-Negative Bacteria::Chlamydiales::Chlamydiaceae::Chlamydia::Chlamydia trachomatis
dc.subject
PHENOMENA AND PROCESSES::Genetic Phenomena::Genotype
dc.subject
DISEASES::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Negative Bacterial Infections::Chlamydiaceae Infections::Chlamydia Infections::Lymphogranuloma Venereum
dc.subject
Other subheadings::Other subheadings::Other subheadings::/epidemiology
dc.subject
ORGANISMOS::Bacteria::bacterias gramnegativas::Chlamydiales::Chlamydiaceae::Chlamydia::Chlamydia trachomatis
dc.subject
FENÓMENOS Y PROCESOS::fenómenos genéticos::genotipo
dc.subject
ENFERMEDADES::infecciones bacterianas y micosis::infecciones bacterianas::infecciones por bacterias gramnegativas::infecciones por Chlamydiaceae::infecciones por Chlamydia::linfogranuloma venéreo
dc.subject
Otros calificadores::Otros calificadores::Otros calificadores::/epidemiología
dc.title
Ongoing evolution of Chlamydia trachomatis lymphogranuloma venereum: exploring the genomic diversity of circulating strains
dc.type
info:eu-repo/semantics/article
dc.type
info:eu-repo/semantics/publishedVersion
