dc.contributor
Institut Català de la Salut
dc.contributor
[Samarkanova D] Banc de Sang i Teixits, 08005 Barcelona, Spain. Grup de Recerca en Medicina Transfusional, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Departament de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Cox S, Hernandez D] Anthony Nolan Research Institute, Royal Free Hospital, London, UK. Barcelona Tissue Bank, Banc de Sang i Teixits, Barcelona, Spain. [Rodriguez L] Banc de Sang i Teixits, 08005 Barcelona, Spain. [Pérez ML] Banc de Sang i Teixits, 08005 Barcelona, Spain. Barcelona Tissue Bank, Banc de Sang i Teixits, Barcelona, Spain. Regenerative Medicine and Tissue Bioengineering Research Group (SGR 1113), Biomedical Research Institute (IIB-Sant Pau), Barcelona, Spain. [Madrigal A] Banc de Sang i Teixits, 08005 Barcelona, Spain. Anthony Nolan Research Institute, Royal Free Hospital, London, UK. UCL Cancer Institute, Royal Free Campus, London, UK. [Querol S] Banc de Sang i Teixits, 08005 Barcelona, Spain. Grup de Recerca en Medicina Transfusional, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain
dc.contributor
Vall d'Hebron Barcelona Hospital Campus
dc.contributor.author
Cox, Steven
dc.contributor.author
Hernandez, Diana
dc.contributor.author
Rodriguez, Luciano
dc.contributor.author
Pérez, Maria Luisa
dc.contributor.author
Madrigal, Alejandro
dc.contributor.author
Querol Giner, Sergi
dc.contributor.author
Samarkanova, Dinara
dc.date.accessioned
2025-10-24T08:55:55Z
dc.date.available
2025-10-24T08:55:55Z
dc.date.issued
2022-03-25T07:23:15Z
dc.date.issued
2022-03-25T07:23:15Z
dc.date.issued
2021-07-02
dc.identifier
Samarkanova D, Cox S, Hernandez D, Rodriguez L, Pérez ML, Madrigal A, et al. Cord blood and amniotic membrane extract eye drop preparations display immune-suppressive and regenerative properties. Sci Rep. 2021 Jul 2;11:13754.
dc.identifier
https://hdl.handle.net/11351/7265
dc.identifier
10.1038/s41598-021-93150-7
dc.identifier
000672163000030
dc.identifier.uri
http://hdl.handle.net/11351/7265
dc.description.abstract
Immunologia; Investigació mèdica
dc.description.abstract
Inmunología; Investigación médica
dc.description.abstract
Immunology; Medical research
dc.description.abstract
Diseases and injuries that compromise the ocular surface cause considerable patient distress and have long term consequences for their quality of life. Treatment modalities that can address the delicate balance of tissue regeneration, inflammation and maintenance of corneal transparency are therefore needed. We have recently formulated two novel eye drops from placental tissues: cord blood platelet lysate (CBED) and amniotic membrane extract eye drops (AMED), which can be used to treat severe ocular disorders. Here we characterise these two preparations by measuring: (a) growth factors (GF) and cytokines composition, (b) promotion of human corneal epithelial cell (HCEC) growth and (c) effects on immune cells in a lymphocyte culture assay. Finally, their bioavailability was assayed in an ex vivo porcine corneal model. We show that both preparations contain GF and cytokines that were able to promote the in vitro growth of HCEC and support repair in an in vitro scratch test. When assessed in a lymphocyte culture, both favoured immune suppression reducing the cellular expression of NKG2D and CD107a as well as the production of interferon gamma (IFN-γ) in natural killer, NKT and T cells. Regarding bioavailability, CBED active molecules were found mainly in the pre-corneal fraction with some penetration into the corneal fraction, in an ex vivo model. In summary, both placental-derived allogeneic preparations, CBED and AMED, display regenerative and immunomodulatory capabilities. These results will help define mechanisms of action and the best indications and doses of each product for use in a particular patient and support the development of off-the-shelf therapies for ocular surface pathologies in which wound healing defects and inflammatory events are contributing factors.
dc.description.abstract
This work was funded by BST intern grant (Ref I. 2017.036), and Anthony Nolan core funding.
dc.format
application/pdf
dc.format
application/pdf
dc.publisher
Nature Research
dc.relation
Scientific Reports;11
dc.relation
https://doi.org/10.1038/s41598-021-93150-7
dc.rights
Attribution 4.0 International
dc.rights
http://creativecommons.org/licenses/by/4.0/
dc.rights
info:eu-repo/semantics/openAccess
dc.subject
Còrnia - Malalties - Tractament
dc.subject
Farmacologia ocular
dc.subject
Sang fetal - Ús terapèutic - Eficàcia
dc.subject
DISEASES::Eye Diseases::Corneal Diseases
dc.subject
Other subheadings::Other subheadings::Other subheadings::/drug therapy
dc.subject
CHEMICALS AND DRUGS::Pharmaceutical Preparations::Solutions::Pharmaceutical Solutions::Ophthalmic Solutions
dc.subject
ANATOMY::Fluids and Secretions::Body Fluids::Blood::Fetal Blood
dc.subject
ENFERMEDADES::oftalmopatías::enfermedades de la córnea
dc.subject
Otros calificadores::Otros calificadores::Otros calificadores::/farmacoterapia
dc.subject
COMPUESTOS QUÍMICOS Y DROGAS::preparados farmacéuticos::soluciones::soluciones farmacéuticas::soluciones oftálmicas
dc.subject
ANATOMÍA::líquidos y secreciones::líquidos corporales::sangre::sangre fetal
dc.title
Cord blood and amniotic membrane extract eye drop preparations display immune-suppressive and regenerative properties
dc.type
info:eu-repo/semantics/article
dc.type
info:eu-repo/semantics/publishedVersion
