dc.contributor
Institut Català de la Salut
dc.contributor
[García-Muñoz E] Banc de Sang i Teixits, 08005 Barcelona, Spain. [Vives J] Banc de Sang i Teixits, 08005 Barcelona, Spain. Grup d'Enginyeria de Teixits Musculoesquelètics, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. Departament de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain
dc.contributor
Vall d'Hebron Barcelona Hospital Campus
dc.contributor.author
García-Muñoz, Elisabeth
dc.contributor.author
Vives Armengol, Joaquim
dc.date.accessioned
2025-10-24T08:51:38Z
dc.date.available
2025-10-24T08:51:38Z
dc.date.issued
2022-02-25T13:50:52Z
dc.date.issued
2022-02-25T13:50:52Z
dc.identifier
García-Muñoz E, Vives J. Towards the standardization of methods of tissue processing for the isolation of mesenchymal stromal cells for clinical use. Cytotechnology. 2021 Jun;73:513–522.
dc.identifier
https://hdl.handle.net/11351/7095
dc.identifier
10.1007/s10616-021-00474-3
dc.identifier
000648804900001
dc.identifier.uri
http://hdl.handle.net/11351/7095
dc.description.abstract
Teixit adipós; Medul·la òssia; Cèl·lules estromals mesenquimals multipotents
dc.description.abstract
Tejido adiposo; Médula ósea; Células estromales mesenquimales multipotentes
dc.description.abstract
Adipose tissue; Bone marrow; Multipotent mesenchymal stromal cells
dc.description.abstract
Multipotent mesenchymal stromal cells (MSCs) are currently the most extensively studied type of adult stem cells in advanced stages of development in the field of regenerative medicine. The biological properties of MSCs have generated great hope for their therapeutic use in degenerative and autoimmune conditions that, at present, lack effective treatment options. Over the last decades, MSCs have been typically obtained from adult bone marrow, but the extraction process is highly invasive and the quality and numbers of isolated cells is drastically influenced by patient age, medication and associated comorbidities. Therefore, there is currently an open discussion on the convenience of allogeneic over autologous treatments, despite potential disadvantages such as rejection by the host. This shift to the allogeneic setting entails the need for high production of MSCs to ensure availability of sufficient cell numbers for transplantation, and therefore making the search for alternative tissue sources of highly proliferative MSC cultures with low levels of senescence occurrence, which is one of the greatest current challenges in the scale up of therapeutic cell bioprocessing. Herein we (i) present the main isolation protocols of MSCs from bone marrow, adipose tissue and Wharton’s jelly of the umbilical cord; and (ii) compare their qualities from a bioprocess standpoint, addressing both quality and regulatory aspects, in view of their anticipated clinical use.
dc.description.abstract
This work has been developed in the context of the Spanish Cell Therapy Network (TerCel, expedient number: RD16/0011/0028) and project PI19/01788, which is funded by Instituto de Salud Carlos III and co-funded by European Union (ERDF/ESF)—A way to build Europe.
dc.format
application/pdf
dc.relation
Cytotechnology;73
dc.relation
https://doi.org/10.1007/s10616-021-00474-3
dc.relation
info:eu-repo/grantAgreement/ES/PE2017-2020/PI19%2F01788
dc.rights
Attribution 4.0 International
dc.rights
http://creativecommons.org/licenses/by/4.0/
dc.rights
info:eu-repo/semantics/openAccess
dc.subject
Sistema nerviós - Degeneració - Tractament
dc.subject
Cèl·lules mare mesenquimàtiques - Ús terapèutic
dc.subject
DISEASES::Nervous System Diseases::Neurodegenerative Diseases
dc.subject
Other subheadings::Other subheadings::/therapy
dc.subject
ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Therapeutics::Biological Therapy::Cell- and Tissue-Based Therapy::Cell Transplantation::Stem Cell Transplantation::Mesenchymal Stem Cell Transplantation
dc.subject
ENFERMEDADES::enfermedades del sistema nervioso::enfermedades neurodegenerativas
dc.subject
Otros calificadores::Otros calificadores::/terapia
dc.subject
TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::terapéutica::terapia biológica::tratamientos basados en células y tejidos::trasplante de células::trasplante de células madre::trasplante de células madre mesenquimatosas
dc.title
Towards the standardization of methods of tissue processing for the isolation of mesenchymal stromal cells for clinical use
dc.type
info:eu-repo/semantics/article
dc.type
info:eu-repo/semantics/publishedVersion
