dc.contributor
Institut Català de la Salut
dc.contributor
[Martínez-Torró C, Torres-Puig S, Sánchez-Alba L, González-Martín M, Marcos-Silva M] Institut de Biotecnologia i Biomedicina and Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Monge M, Canals F] Vall d’Hebron Institute of Oncology (VHIO), Barcelona, Spain
dc.contributor
Vall d'Hebron Barcelona Hospital Campus
dc.contributor.author
Martínez-Torró, Carlos
dc.contributor.author
Torres-Puig, Sergi
dc.contributor.author
Monge Azemar, Marta
dc.contributor.author
Sánchez-Alba, Lucía
dc.contributor.author
González-Martín, Miguel
dc.contributor.author
Marcos-Silva, Marina
dc.contributor.author
Canals Suris, Francesc
dc.date.accessioned
2025-10-25T05:38:24Z
dc.date.available
2025-10-25T05:38:24Z
dc.date.issued
2021-09-09T06:20:30Z
dc.date.issued
2021-09-09T06:20:30Z
dc.date.issued
2020-12-20
dc.identifier
Martínez-Torró C, Torres-Puig S, Monge M, Sánchez-Alba L, González-Martín M, Marcos-Silva M, et al. Transcriptional response to metal starvation in the emerging pathogen Mycoplasma genitalium is mediated by Fur-dependent and –independent regulatory pathways. Emerg Microbes Infect. 2020 Dec 20;9:5-9.
dc.identifier
https://hdl.handle.net/11351/6284
dc.identifier
10.1080/22221751.2019.1700762
dc.identifier
000510381200002
dc.identifier.uri
http://hdl.handle.net/11351/6284
dc.description.abstract
Regulador d'absorció fèrric; Mycoplasma genitalium; Patogen emergent d'ITS
dc.description.abstract
Regulador de la absorción férrico; Mycoplasma genitalium; Patógeno emergente de ITS
dc.description.abstract
Ferric uptake regulator; Mycoplasma genitalium; Emerging STI pathogen
dc.description.abstract
Transition metals participate in numerous enzymatic reactions and they are essential for survival in all living organisms. For this reason, bacterial pathogens have evolved dedicated machineries to effectively compete with their hosts and scavenge metals at the site of infection. In this study, we investigated the mechanisms controlling metal acquisition in the emerging human pathogen Mycoplasma genitalium. We observed a robust transcriptional response to metal starvation, and many genes coding for predicted lipoproteins and ABC-transporters were significantly up-regulated. Transcriptional analysis of a mutant strain lacking a metalloregulator of the Fur family revealed the activation of a full operon encoding a putative metal transporter system and a gene coding for a Histidine-rich lipoprotein (Hrl). We recognized a conserved sequence with dyad symmetry within the promoter region of the Fur-regulated genes. Mutagenesis of the predicted Fur operator within the hrl promoter abrogated Fur- and metal-dependent expression of a reporter gene. Metal starvation still impelled a strong transcriptional response in the fur mutant, demonstrating the existence of Fur-independent regulatory pathways controlling metal homeostasis. Finally, analysis of metal accumulation in the wild-type strain and the fur mutant by ICP-MS revealed an important role of Fur in nickel acquisition.
dc.description.abstract
This work was supported by the Ministerio de Ciencia, Innovación y Universidades (Grant BIO2017-84166-R). The Proteomics Unit at VHIO is a member ProteoRed, PRB3 (Grant IPT17/0019 – ISCIII-SGEFI/ERDF).
dc.format
application/pdf
dc.format
application/pdf
dc.format
application/pdf
dc.publisher
Taylor and Francis
dc.relation
Emerging Microbes & Infections;9
dc.relation
https://doi.org/10.1080/22221751.2019.1700762
dc.relation
info:eu-repo/grantAgreement/ES/PE2013-2016/BIO2017-84166-R
dc.rights
Attribution 4.0 International
dc.rights
http://creativecommons.org/licenses/by/4.0/
dc.rights
info:eu-repo/semantics/openAccess
dc.subject
Regulació genètica
dc.subject
Malalties bacterianes gramnegatives
dc.subject
ORGANISMS::Bacteria::Gram-Negative Bacteria::Tenericutes::Mycoplasmatales::Mycoplasmataceae::Mycoplasma::Mycoplasma genitalium
dc.subject
PHENOMENA AND PROCESSES::Genetic Phenomena::Gene Expression Regulation::Gene Expression Regulation, Bacterial
dc.subject
ORGANISMOS::Bacteria::bacterias gramnegativas::Tenericutes::Mycoplasmatales::Mycoplasmataceae::Mycoplasma::Mycoplasma genitalium
dc.subject
FENÓMENOS Y PROCESOS::fenómenos genéticos::regulación de la expresión génica::regulación de la expresión génica bacteriana
dc.title
Transcriptional response to metal starvation in the emerging pathogen Mycoplasma genitalium is mediated by Fur-dependent and –independent regulatory pathways
dc.type
info:eu-repo/semantics/article
dc.type
info:eu-repo/semantics/publishedVersion
