dc.contributor
Institut Català de la Salut
dc.contributor
[Segura MF] Department of Pathology, New York University School of Medicine, New York, NY, USA. Interdisciplinary Melanoma Cooperative Group, New York University Perlmutter Cancer Institute, NYU School of Medicine, New York, NY, USA. Grup Recerca translacional amb càncer infantil, Vall d'Hebron Institut de Rercerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. [Jubierre L, Soriano A, Masanas M] Grup Recerca translacional amb càncer infantil, Vall d'Hebron Institut de Rercerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. [Li S] Departments of Structural and Chemical Biology, Genetics and Genomic Sciences and Pediatrics, Icahn School of Medicine at Mount Sinai, New York, NY, USA. [Koetz L, Gaziel-Sovran A] Department of Pathology, New York University School of Medicine, New York, NY, USA. Interdisciplinary Melanoma Cooperative Group, New York University Perlmutter Cancer Institute, NYU School of Medicine, New York, NY, USA
dc.contributor
Vall d'Hebron Barcelona Hospital Campus
dc.contributor.author
Segura Ginard, Miguel
dc.contributor.author
Jubierre Zapater, Luz
dc.contributor.author
Li, SiDe
dc.contributor.author
Soriano Fernández, Aroa
dc.contributor.author
Koetz, Lisa
dc.contributor.author
Gaziel-Sovran, Avital
dc.contributor.author
Masanas Jimenez, Marc
dc.date.accessioned
2025-10-24T08:48:13Z
dc.date.available
2025-10-24T08:48:13Z
dc.date.issued
2021-04-27T09:53:12Z
dc.date.issued
2021-04-27T09:53:12Z
dc.date.issued
2017-04-18
dc.identifier
Segura MF, Jubierre L, Li S, Soriano A, Koetz L, Gaziel-Sovran A, et al. Krüppel-like factor 4 (KLF4) regulates the miR-183~96~182 cluster under physiologic and pathologic conditions. Oncotarget. 2017 Apr 18;8(16):26298–311.
dc.identifier
https://hdl.handle.net/11351/5909
dc.identifier
10.18632/oncotarget.15459
dc.identifier
000399819700042
dc.identifier.uri
http://hdl.handle.net/11351/5909
dc.description.abstract
Cèl·lules mare embrionàries; Melanoma; MicroARN
dc.description.abstract
Células madre embrionarias; Melanoma; MicroARN
dc.description.abstract
Embryonic stem cells; Melanoma; MicroRNA
dc.description.abstract
MicroRNAs (miRNAs) are a class of endogenous non-coding small RNAs that post-transcriptionally control the translation and stability of target mRNAs in a sequence-dependent manner. MiRNAs are essential for key cellular processes including proliferation, differentiation, cell death and metabolism, among others. Consequently, alterations of miRNA expression contribute to developmental defects and a myriad of diseases.
The expression of miRNAs can be altered by several mechanisms including gene copy number alterations, aberrant DNA methylation, defects of the miRNA processing machinery or unscheduled expression of transcription factors. In this work, we sought to analyze the regulation of the miR-182 cluster, located at the 7q32 locus, which encodes three different miRNAs that are abundantly expressed in human embryonic stem cells and de-regulated in cancer. We have found that the Krüppel-like factor 4 (KLF4) directly regulates miR-182 cluster expression in human embryonic stem cells (hESCs) and in melanoma tumors, in which the miR-182 cluster is highly expressed and has a pro-metastatic role. Furthermore, higher KLF4 expression was found to be associated with metastatic progression and poor patient outcome. Loss of function experiments revealed that KLF4 is required for melanoma cell maintenance. These findings provide new insights into the regulation of the miR-182 cluster expression and new opportunities for therapeutic intervention in tumors in which the KLF4-miR-182 cluster axis is deregulated.
dc.description.abstract
This work was supported by NCI/NIH Grant (5R01CA155234), Instituto de Salud Carlos III (CP11/00052 and RD12/0036/0016) co-financed by the European Regional Development Fund (ERDF), and European Commission’s Framework Programme 7 through the Marie Curie Career Integration Grants.
dc.format
application/pdf
dc.publisher
Impact Journals
dc.relation
Oncotarget;8(16)
dc.relation
https://www.oncotarget.com/article/15459/text/
dc.relation
info:eu-repo/grantAgreement/ES/1PN/2008-2011/CP11%2F00052
dc.relation
info:eu-repo/grantAgreement/ES/2PN/2008-2011/RD12%2F0036%2F0016
dc.rights
Attribution 4.0 International
dc.rights
http://creativecommons.org/licenses/by/4.0/
dc.rights
info:eu-repo/semantics/openAccess
dc.subject
Cèl·lules mare embrionàries
dc.subject
Factors de transcripció
dc.subject
ANATOMY::Cells::Stem Cells::Pluripotent Stem Cells::Embryonic Stem Cells::Human Embryonic Stem Cells
dc.subject
DISEASES::Neoplasms
dc.subject
CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Proteins::DNA-Binding Proteins::Kruppel-Like Transcription Factors
dc.subject
ANATOMÍA::células::células madre::células madre pluripotentes::células madre embrionarias::células madre embrionarias humanas
dc.subject
ENFERMEDADES::neoplasias
dc.subject
COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::proteínas::proteínas de unión al ADN::factores de transcripción similares a Kruppel
dc.title
Krüppel-like factor 4 (KLF4) regulates the miR-183~96~182 cluster under physiologic and pathologic conditions
dc.type
info:eu-repo/semantics/article
dc.type
info:eu-repo/semantics/publishedVersion
