dc.contributor
Institut Català de la Salut
dc.contributor
[Scharf MW, Forsgård RA, Prado SBR, Repsilber D, Brummer R] Nutrition-Gut-Brain Interactions Research Centre, School of Medical Sciences, Faculty of Medicine and Health, Örebro University, Örebro, Sweden. [Ganda Mall JP] Nutrition-Gut-Brain Interactions Research Centre, School of Medical Sciences, Faculty of Medicine and Health, Örebro University, Örebro, Sweden. Grup de Recerca de Fisiologia i Fisiopatologia Digestiva, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain
dc.contributor
Vall d'Hebron Barcelona Hospital Campus
dc.contributor.author
Scharf, Mathias Wolfgang
dc.contributor.author
Forsgård, Richard
dc.contributor.author
Prado, Samira
dc.contributor.author
Ganda Mall, John-Peter
dc.contributor.author
Repsilber, Dirk
dc.contributor.author
Brummer, Robert
dc.date.accessioned
2025-10-25T08:54:03Z
dc.date.available
2025-10-25T08:54:03Z
dc.date.issued
2025-10-22T12:19:14Z
dc.date.issued
2025-10-22T12:19:14Z
dc.identifier
Scharf MW, Forsgård RA, Prado SBR, Ganda Mall JP, Repsilber D, Brummer RJ, et al. Acute effects of butyrate on intestinal permeability in patients with irritable bowel syndrome assessed by a novel colonoscopy research model. Gut Microbes. 2025;17(1):2545414.
dc.identifier
http://hdl.handle.net/11351/13915
dc.identifier
10.1080/19490976.2025.2545414
dc.identifier
001550238000001
dc.identifier.uri
http://hdl.handle.net/11351/13915
dc.description.abstract
Ussing chamber; In vivo; Intestinal barrier function
dc.description.abstract
Cámara de Ussing; In vivo; Función de barrera intestinal
dc.description.abstract
Cambra d'Ussing; In vivo; Funció de barrera intestinal
dc.description.abstract
Irritable bowel syndrome (IBS) is a prevalent gastrointestinal disorder for which effective treatment strategies are insufficient. Butyrate, a microbiota-derived short-chain fatty acid believed to strengthen the intestinal barrier function, might be a potential new treatment option. This study aimed to investigate potential protective effects of acute in vivo butyrate exposure on intestinal barrier function in healthy subjects and patients with IBS. For this, we used an experimental colonoscopy-perfusion model for colon-specific butyrate delivery and adequate tissue sampling. Seventeen IBS and 17 healthy subjects underwent a colonoscopy procedure exposing a predefined colonic area to 100 mmol/L butyrate for 90 min in vivo. Mucosal biopsies collected pre- and post-butyrate exposure were stimulated in Ussing chambers with/without sodium deoxycholate (DC) to induce intestinal hyperpermeability. Intestinal permeability was measured by fluorescein isothiocyanate-dextran and horseradish peroxidase passage. DC-stimulation significantly increased para- and transcellular permeability in biopsies collected pre-butyrate exposure. DC-induced transcellular hyperpermeability was significantly alleviated in biopsies collected post-butyrate exposure compared to pre-exposure in patients with IBS (p = 0.034). In conclusion, we established a colonoscopy research model for colon-specific delivery and sampling and demonstrated acute protective effects of butyrate on transcellular intestinal permeability in patients with IBS. The results support butyrate's potential role in novel treatment strategies in IBS. Clinicaltrials.gov number: NCT05249023.
dc.description.abstract
This work was supported by the Swedish Research Council under grant number [2017-02694], EFSD/Novo Nordisk Programme 2017 and Lantmännen R&D under grant number [2019F010].
dc.format
application/pdf
dc.publisher
Taylor & Francis
dc.relation
Gut Microbes;17(1)
dc.relation
https://doi.org/10.1080/19490976.2025.2545414
dc.rights
Attribution 4.0 International
dc.rights
http://creativecommons.org/licenses/by/4.0/
dc.rights
info:eu-repo/semantics/openAccess
dc.subject
Intestins - Permeabilitat
dc.subject
Còlon irritable - Tractament
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Mucosa intestinal - Efecte dels medicaments
dc.subject
DISEASES::Digestive System Diseases::Gastrointestinal Diseases::Intestinal Diseases::Colonic Diseases::Colonic Diseases, Functional::Irritable Bowel Syndrome
dc.subject
Other subheadings::Other subheadings::Other subheadings::/drug therapy
dc.subject
PHENOMENA AND PROCESSES::Chemical Phenomena::Permeability
dc.subject
ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Techniques, Digestive System::Endoscopy, Digestive System::Endoscopy, Gastrointestinal::Colonoscopy
dc.subject
ANATOMY::Digestive System::Gastrointestinal Tract::Intestines::Intestinal Mucosa
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Other subheadings::Other subheadings::/drug effects
dc.subject
CHEMICALS AND DRUGS::Organic Chemicals::Carboxylic Acids::Acids, Acyclic::Butyrates
dc.subject
ENFERMEDADES::enfermedades del sistema digestivo::enfermedades gastrointestinales::enfermedades intestinales::enfermedades del colon::enfermedades funcionales del colon::síndrome del colon irritable
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Otros calificadores::Otros calificadores::Otros calificadores::/farmacoterapia
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FENÓMENOS Y PROCESOS::fenómenos químicos::permeabilidad
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TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::diagnóstico::técnicas y procedimientos diagnósticos::técnicas diagnósticas digestivas::endoscopia digestiva::endoscopia gastrointestinal::colonoscopia
dc.subject
ANATOMÍA::sistema digestivo::tracto gastrointestinal::intestinos::mucosa intestinal
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Otros calificadores::Otros calificadores::/efectos de los fármacos
dc.subject
COMPUESTOS QUÍMICOS Y DROGAS::compuestos orgánicos::ácidos carboxílicos::ácidos acíclicos::butiratos
dc.title
Acute effects of butyrate on intestinal permeability in patients with irritable bowel syndrome assessed by a novel colonoscopy research model
dc.type
info:eu-repo/semantics/article
dc.type
info:eu-repo/semantics/publishedVersion
