dc.contributor
Institut Català de la Salut
dc.contributor
[Giralt M, Ferrer R, Díaz-Troyano N] Servei de Bioquímica, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Grup de Bioquímica Clínica, Vehiculització de Fàrmacs i Teràpia, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain. [Vega B, Luque-Ramírez M] Department of Endocrinology & Nutrition, Hospital Universitario Ramón y Cajal & Universidad de Alcalá & Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS) & Centro. [Martínez S] Servei d’Anàlisis Clíniques i Bioquímica, Hospital Germans Trias i Pujol, Barcelona, Spain. [Cardona I] Servei de Farmàcia, Vall d’Hebron Hospital Universitari, Barcelona, Spain
dc.contributor
Vall d'Hebron Barcelona Hospital Campus
dc.contributor.author
Vega, Belen
dc.contributor.author
Luque-Ramírez, Manuel
dc.contributor.author
Martínez Couselo, Silvia
dc.contributor.author
Cardona, Ignacio
dc.contributor.author
Giralt Arnaiz, Marina
dc.contributor.author
Ferrer Costa, Roser
dc.contributor.author
Díaz-Troyano, Noelia
dc.date.accessioned
2025-10-01T01:27:12Z
dc.date.available
2025-10-01T01:27:12Z
dc.date.issued
2025-09-22T07:18:07Z
dc.date.issued
2025-09-22T07:18:07Z
dc.identifier
Giralt M, Ferrer R, Díaz-Troyano N, Vega B, Luque-Ramírez M, Martínez S, et al. Analytical Interference of Exemestane With Androstenedione Immunoassays. Ann Lab Med. 2025 Jul;45(4):410-419.
dc.identifier
http://hdl.handle.net/11351/13702
dc.identifier
10.3343/alm.2024.0362
dc.identifier.uri
http://hdl.handle.net/11351/13702
dc.description.abstract
Exemestane; Immunoassay; Liquid chromatography-mass spectrometry
dc.description.abstract
Exemestano; Inmunoensayo; Cromatografía líquida-espectrometría de masas
dc.description.abstract
Exemestà; Immunoassaig; Cromatografia líquida-espectrometria de masses
dc.description.abstract
Background: Exemestane, an aromatase inhibitor commonly used for breast cancer treatment, shares structural similarities with sex steroids analyzed in clinical laboratories. We aimed to investigate the influence of exemestane cross-reactivity in the measurement of sex steroids across various immunoassays.
Methods: We conducted a multicenter study involving measurements of androstenedione, testosterone, estradiol, progesterone, and 17-hydroxyprogesterone in serum samples from women undergoing exemestane therapy (N=15; 25 mg/day). Measurements were performed using liquid chromatography-mass spectrometry (LC-MS) and various commercially available chemiluminescence immunoassays, ELISA, and radioimmunoassay. In-vitro cross-reactivity was assessed by adding exemestane and 17-hydroexemestane to serum samples.
Results: Patients undergoing exemestane therapy had markedly falsely elevated androstenedione results in all immunoassays evaluated (N=4), which correlated with serum exemestane levels. In-vitro experiments confirmed this interference to be caused by cross-reactivity with exemestane. Additionally, one immunoassay yielded falsely elevated estradiol results in 20% of patients. However, in-vitro experiments did not confirm this to be caused by cross-reactivity with exemestane or 17-hydroexemestane.
Conclusions: Exemestane cross-reacts with androstenedione immunoassays, causing falsely elevated results in treated patients. This analytical interference may raise unnecessary concerns, leading to expensive diagnostic workups.
dc.format
application/pdf
dc.publisher
Korean Society for Laboratory Medicine
dc.relation
Annals of Laboratory Medicine;45(4)
dc.relation
https://doi.org/10.3343/alm.2024.0362
dc.rights
Attribution-NonCommercial 4.0 International
dc.rights
http://creativecommons.org/licenses/by-nc/4.0/
dc.rights
info:eu-repo/semantics/openAccess
dc.subject
Mama - Càncer - Tractament
dc.subject
Enzims - Inhibidors - Ús terapèutic
dc.subject
Cromatografia de líquids
dc.subject
CHEMICALS AND DRUGS::Chemical Actions and Uses::Pharmacologic Actions::Molecular Mechanisms of Pharmacological Action::Enzyme Inhibitors::Steroid Synthesis Inhibitors::Aromatase Inhibitors
dc.subject
Other subheadings::Other subheadings::/therapeutic use
dc.subject
DISEASES::Neoplasms::Neoplasms by Site::Breast Neoplasms
dc.subject
Other subheadings::Other subheadings::Other subheadings::/drug therapy
dc.subject
ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Investigative Techniques::Immunologic Techniques::Immunoassay
dc.subject
ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Investigative Techniques::Chemistry Techniques, Analytical::Chromatography::Chromatography, Liquid
dc.subject
COMPUESTOS QUÍMICOS Y DROGAS::acciones y usos químicos::acciones farmacológicas::mecanismos moleculares de acción farmacológica::inhibidores enzimáticos::inhibidores de la síntesis esteroidea::inhibidores de la aromatasa
dc.subject
Otros calificadores::Otros calificadores::/uso terapéutico
dc.subject
ENFERMEDADES::neoplasias::neoplasias por localización::neoplasias de la mama
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Otros calificadores::Otros calificadores::Otros calificadores::/farmacoterapia
dc.subject
TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::técnicas de investigación::técnicas inmunológicas::inmunoanálisis
dc.subject
TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::técnicas de investigación::técnicas de química analítica::cromatografía::cromatografía líquida
dc.title
Analytical Interference of Exemestane With Androstenedione Immunoassays
dc.type
info:eu-repo/semantics/article
dc.type
info:eu-repo/semantics/publishedVersion
