Sistema de Salut de Catalunya
Institut Català de la Salut
[Jimenez Catalan JJ] Preclinical Imaging Platform, Vall d’Hebron Institute of Oncology (VHIO), Barcelona, Spain. [Ferrer Clotas M] Gnotobiotic Core and Mouse Metabolism Core, University of California San Francisco, San Francisco, CA, USA. [Camara Serrano JA] Preclinical Therapeutics Core, University of California San Francisco, San Francisco, CA, USA
Vall d'Hebron Barcelona Hospital Campus
2025-09-16T11:01:47Z
2025-09-16T11:01:47Z
2025-06
Mice; Organ volume; Ultrasound
Ratolins; Volum d'òrgans; Ecografia
Ratones; Volumen de órganos; Ecografía
In this work, the authors compared microCT and in vivo ultrasonography in terms of accuracy and efficacy for measuring the volume of various organs in mice. Two quantification protocols were applied: ellipsoidal volume measuring maximum diameters in all three axes in both imaging systems and manual delineation of organ borders in microCT studies. The results were compared with ex vivo volumes. In general, both imaging techniques and quantification protocols are accurate, but ultrasound is faster in both acquisition and analysis. The only accurate method for heart volume measurement is manual segmentation on microCT. For the ovary, none of the techniques and protocols had a positive correlation with ex vivo volume. The three-diameter method can be used for ellipsoid organs because of its rapidity, but for more irregular structures, manual segmentation is recommended, although it is time-consuming.
Article
Published version
English
Rates (Animals de laboratori); Òrgans (Anatomia); Ecografia; Tomografia; ORGANISMS::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Eutheria::Rodentia::Muridae::Murinae::Mice; ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Imaging::Ultrasonography; ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Imaging::Radiography::Tomography, X-Ray::Tomography, X-Ray Computed::X-Ray Microtomography; ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Diagnosis::Diagnostic Techniques and Procedures::Physical Examination::Body Constitution::Body Weights and Measures::Organ Size; ORGANISMOS::Eukaryota::animales::Chordata::vertebrados::mamíferos::Eutheria::Rodentia::Muridae::Murinae::ratones; TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::diagnóstico::técnicas y procedimientos diagnósticos::diagnóstico por imagen::ecografía; TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::diagnóstico::técnicas y procedimientos diagnósticos::diagnóstico por imagen::radiografía::tomografía de rayos X::tomografía computarizada por rayos X::microtomografía de rayos X; TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::diagnóstico::técnicas y procedimientos diagnósticos::exploración física::constitución corporal::pesos y medidas corporales::tamaño de los órganos
MDPI
Journal of Imaging;11(6)
https://doi.org/10.3390/jimaging11060200
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
