dc.contributor
Institut Català de la Salut
dc.contributor
[Vidaur L] Intensive Care Unit, Donostia University Hospital, Donostia-San Sebastián, Spain. Biogipuzkoa, Infectious Diseases Area, Respiratory Infection and Antimicrobial Resistance Group, Osakidetza Basque Health Service, Donostialdea Integrated Health Organization, Microbiology Department, Donostia-San Sebastian, Spain. Centro de Investigacion Biomedica en Red de Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Madrid, Spain. [Guridi A, Leizaola O, Marin J] Intensive Care Unit, Donostia University Hospital, Donostia-San Sebastián, Spain. [Rello J] Centro de Investigacion Biomedica en Red de Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Madrid, Spain. Grup de Recerca Clínica/Innovació en la Pneumònia i Sèpsia, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. IMAGINE, UR-UM107, University of Montpellier, Division of Anaesthesia Critical Care, Pain and Emergency Medicine Nimes University Hospital, Nimes, France. [Sarasqueta C] Clinical Epidemiology Department, OSID Donostialdea. Biogipuzkoa, San Sebastian, Spain
dc.contributor
Vall d'Hebron Barcelona Hospital Campus
dc.contributor.author
Vidaur, Loreto
dc.contributor.author
Guridi, Amalur
dc.contributor.author
Leizaola, Oihana
dc.contributor.author
Marin, Jokin
dc.contributor.author
Rello, Jordi
dc.contributor.author
Sarasqueta, Cristina
dc.date.accessioned
2025-10-24T08:54:56Z
dc.date.available
2025-10-24T08:54:56Z
dc.date.issued
2025-06-16T07:02:34Z
dc.date.issued
2025-06-16T07:02:34Z
dc.date.issued
2025-05-05
dc.identifier
Vidaur L, Guridi A, Leizaola O, Marin J, Rello J, Sarasqueta C, et al. Respiratory dysbiosis as prognostic biomarker of disease severity for adults with community-acquired pneumonia requiring mechanical ventilation. Pneumonia. 2025 May 5;17:10.
dc.identifier
http://hdl.handle.net/11351/13291
dc.identifier
10.1186/s41479-025-00163-1
dc.identifier
001481202600001
dc.identifier.uri
http://hdl.handle.net/11351/13291
dc.description.abstract
Biomarkers; Lung microbiome; Severe community-acquired pneumonia
dc.description.abstract
Biomarcadores; Microbioma pulmonar; Neumonía grave adquirida en la comunidad
dc.description.abstract
Biomarcadors; Microbioma pulmonar; Pneumònia greu adquirida a la comunitat
dc.description.abstract
Objetives
To ascertain the role of the lung microbiome in the development of severe pneumonia and its potential as a biomarker for disease progression.
Methods
BAL samples from 34 adults with severe community-acquired pneumonia (CAP) (17 viral, 8 viral coinfected with bacteria and 9 bacterial) admitted to the ICU for acute respiratory failure between 2019 and 2021 were collected within the first 48 h of admission to the ICU. The microbiome was characterized via the Ion 16S Metagenomics Kit and the Ion Torrent sequencing platform. Clinical factors, including survival, mechanical ventilation duration, blood biomarkers and organ failure in terms of acute respiratory distress syndrome (ARDS), shock or acute renal failure, were correlated with microbiome characteristics.
Results
The microbiome diversity in patients with viral pneumonia was significantly greater than that in patients with bacterial or coinfected pneumonia: the Shannon diversity index was 3.75 (Q1–Q3: 2.5–4.1) versus 0.4 (Q1–Q3: 0.2–1.3) and 0.48 (Q1–Q3: 0.3–1.1), respectively (p < 0.05). The microbiome diversity index was associated with severity-of-illness (APACHE II), independent of the etiology of pneumonia (B coefficient -1.845; p < 0.01). Patients with severe viral CAP who developed ARDS had a lower presence of Proteobacteria, and those who were complicated with ventilator-associated pneumonia had a higher prevalence of Acinetobacter at admission. The mortality of patients with bacterial or coinfected pneumonia was 35%. In coinfected patients, the diversity index was associated with the development of shock.
Conclusion
Patients with severe CAP have low respiratory microbiome diversity, indicating that respiratory microbiome diversity is a potential biomarker of disease severity.
dc.description.abstract
This study was supported by a grant from the Spanish Ministry of Health, Instituto de Salud Carlos III, FIS17/01463.
dc.format
application/pdf
dc.relation
https://doi.org/10.1186/s41479-025-00163-1
dc.rights
Attribution-NonCommercial-NoDerivatives 4.0 International
dc.rights
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights
info:eu-repo/semantics/openAccess
dc.subject
Pneumònia adquirida a la comunitat - Prognosi
dc.subject
Marcadors bioquímics
dc.subject
Respiració artificial
dc.subject
DISEASES::Pathological Conditions, Signs and Symptoms::Pathologic Processes::Dysbiosis
dc.subject
CHEMICALS AND DRUGS::Biological Factors::Biomarkers
dc.subject
ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Diagnosis::Prognosis
dc.subject
DISEASES::Bacterial Infections and Mycoses::Infection::Community-Acquired Infections
dc.subject
DISEASES::Respiratory Tract Diseases::Lung Diseases::Pneumonia
dc.subject
ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Therapeutics::Airway Management::Respiration, Artificial
dc.subject
ENFERMEDADES::afecciones patológicas, signos y síntomas::procesos patológicos::disbacteriosis
dc.subject
COMPUESTOS QUÍMICOS Y DROGAS::factores biológicos::biomarcadores
dc.subject
TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::diagnóstico::pronóstico
dc.subject
ENFERMEDADES::infecciones bacterianas y micosis::infección::infecciones adquiridas en la comunidad
dc.subject
ENFERMEDADES::enfermedades respiratorias::enfermedades pulmonares::neumonía
dc.subject
TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::terapéutica::manejo de la via aérea::respiración artificial
dc.title
Respiratory dysbiosis as prognostic biomarker of disease severity for adults with community-acquired pneumonia requiring mechanical ventilation
dc.type
info:eu-repo/semantics/article
dc.type
info:eu-repo/semantics/publishedVersion
