dc.contributor
Institut Català de la Salut
dc.contributor
[Cano Á, Yubero ML, Millá C, Puerto-Belda V, Ruz JJ, Kosaka PM] Bionanomechanics Lab, Instituto de Micro y Nanotecnología, IMN-CNM (CSIC), Tres Cantos, Madrid, Spain. [Malumbres M] Cancer Cell Cycle Group, Vall d’Hebron Institute of Oncology (VHIO), Barcelona, Spain
dc.contributor
Vall d'Hebron Barcelona Hospital Campus
dc.contributor.author
Cano, Álvaro
dc.contributor.author
L. Yubero, Marina P.
dc.contributor.author
Millá, Carmen
dc.contributor.author
Puerto-Belda, Verónica
dc.contributor.author
Ruz Martinez, Jose Jaime
dc.contributor.author
M. Kosaka, Priscila
dc.contributor.author
Malumbres, Marcos
dc.date.accessioned
2025-10-25T05:38:31Z
dc.date.available
2025-10-25T05:38:31Z
dc.date.issued
2025-01-10T13:11:40Z
dc.date.issued
2025-01-10T13:11:40Z
dc.date.issued
2024-11-15
dc.identifier
Cano Á, Yubero ML, Millá C, Puerto-Belda V, Ruz JJ, Kosaka PM, et al. Rapid mechanical phenotyping of breast cancer cells based on stochastic intracellular fluctuations. iScience. 2024 Nov 15;27(11):110960.
dc.identifier
https://hdl.handle.net/11351/12394
dc.identifier
10.1016/j.isci.2024.110960
dc.identifier
001338900500001
dc.identifier.uri
http://hdl.handle.net/11351/12394
dc.description.abstract
Cancer; Cancer systems biology
dc.description.abstract
Càncer; Biologia dels sistemes del càncer
dc.description.abstract
Cáncer; Biología de sistemas del cáncer
dc.description.abstract
Predicting the phenotypic impact of genetic variants and treatments is crucial in cancer genetics and precision oncology. Here, we have developed a noise decorrelation method that enables quantitative phase imaging (QPI) with the capability for label-free noninvasive mapping of intracellular dry mass fluctuations within the millisecond-to-second timescale regime, previously inaccessible due to temporal phase noise. Applied to breast cancer cells, this method revealed regions driven by thermal forces and regions of intense activity fueled by ATP hydrolysis. Intriguingly, as malignancy increases, the cells strategically expand these active regions to satisfy increasing energy demands. We propose parameters encapsulating key information about the spatiotemporal distribution of intracellular fluctuations, enabling precise phenotyping. This technique addresses the need for accurate, rapid functional screening methods in cancer medicine.
dc.description.abstract
This work was partially supported by the Spanish Science and Innovation Ministry through projects RTI2018-099369-B-I00 (related doctoral fellowship [FPI] PRE2019-087448), AES (DTS21/00136)/ISCIII/EU/FEDER, “CELLBIOPHYS” (PID2021-126993OB-I00/MCIN/AEI/10.13039/501100011033/FEDER, UE), “ONCOLIGHT” (PDC2022-133503- I00/MCIN/AEI/10.13039/501100011033/European Union/Next Generation EU/PRTR), and “ONCODEEPLASM” (PLEC2021- 007892/MCIN/AEI/10.13039/501100011033/European Union/Next Generation EU/PRTR) and by Comunidad Autónoma de Madrid (Y2020/BIO- 65194 scCANCER-CM). This work has also been supported by private FERO Foundation and Elena Torres Cancer Association funding. We acknowledge the service from the Micro and Nanofabrication Laboratory and X-SEM laboratory at IMN-CNM funded by the Comunidad Autónoma de Madrid (project S2018/NMT-4291 TEC2SPACE) and by MINECO (project CSIC13-4E-1794 with support from FEDER, FSE).
dc.format
application/pdf
dc.relation
iScience;27(11)
dc.relation
https://doi.org/10.1016/j.isci.2024.110960
dc.rights
Attribution 4.0 International
dc.rights
http://creativecommons.org/licenses/by/4.0/
dc.rights
info:eu-repo/semantics/openAccess
dc.subject
Mama - Càncer - Aspectes genètics
dc.subject
Imatges - Processament
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Cèl·lules canceroses
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PHENOMENA AND PROCESSES::Genetic Phenomena::Phenotype
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DISEASES::Neoplasms::Neoplasms by Site::Breast Neoplasms
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Other subheadings::Other subheadings::Other subheadings::/genetics
dc.subject
Image Processing Computer Assisted
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ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Imaging::Microscopy::Microscopy, Interference::Microscopy, Phase-Contrast
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FENÓMENOS Y PROCESOS::fenómenos genéticos::fenotipo
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ENFERMEDADES::neoplasias::neoplasias por localización::neoplasias de la mama
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Otros calificadores::Otros calificadores::Otros calificadores::/genética
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CIENCIA DE LA INFORMACIÓN::Ciencias de la información::metodologías computacionales::procesamiento de imágenes asistido por ordenador
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TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::diagnóstico::técnicas y procedimientos diagnósticos::diagnóstico por imagen::microscopía::microscopía de interferencia::microscopía de contraste de fases
dc.title
Rapid mechanical phenotyping of breast cancer cells based on stochastic intracellular fluctuations
dc.type
info:eu-repo/semantics/article
dc.type
info:eu-repo/semantics/publishedVersion
