dc.contributor
Institut Català de la Salut
dc.contributor
[López-Martínez M] Servei de Nefrologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Departament de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Armengol MP, Pey I, Farré X] Genomic Platform, Germans Trias i Pujol’s Research Institute, Badalona, Barcelona, Spain. [Rodríguez-Martínez P] Pathology Department, Germans Trias i Pujol Hospital, Badalona, Barcelona, Spain. [Ferrer M] Unitat d’Estadística i Bioinformàtica, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain
dc.contributor
Vall d'Hebron Barcelona Hospital Campus
dc.contributor.author
Armengol, Maria del Pilar
dc.contributor.author
Pey, Irina
dc.contributor.author
Farré, Xavier
dc.contributor.author
Rodríguez-Martínez, Paula
dc.contributor.author
Ferrer Almirall, Mireia
dc.contributor.author
López-Martínez, Marina
dc.date.accessioned
2024-11-01T02:58:05Z
dc.date.available
2024-11-01T02:58:05Z
dc.date.issued
2024-07-04T06:28:35Z
dc.date.issued
2024-07-04T06:28:35Z
dc.date.issued
2024-06-11
dc.identifier
López-Martínez M, Armengol MP, Pey I, Farré X, Rodríguez-Martínez P, Ferrer M, et al. Integrated miRNA–mRNA Analysis Reveals Critical miRNAs and Targets in Diet-Induced Obesity-Related Glomerulopathy. Int J Mol Sci. 2024 Jun 11;25(12):6437.
dc.identifier
https://hdl.handle.net/11351/11662
dc.identifier
10.3390/ijms25126437
dc.identifier.uri
http://hdl.handle.net/11351/11662
dc.description.abstract
Wistar rats; MicroRNA; Glomerulopathy
dc.description.abstract
Rates Wistar; MicroARN; Glomerulopatia
dc.description.abstract
Ratas Wistar; MicroARN; Glomerulopatía
dc.description.abstract
This study aimed to investigate obesity-related glomerulopathy (ORG) at cellular, structural, and transcriptomic levels. Thirty Wistar rats were randomized into two groups: 15 rats were fed with a standard diet (SD-rats), and 15 rats were fed with a high-fat diet (HFD-rats). After 10 weeks, the weight, kidney function, histological features, and transcriptomic changes were assessed. HFD-rats gained significantly more weight (55.8% vs. 29.2%; p < 0.001) and albuminuria (10,384.04 ng/mL vs. 5845.45 ng/mL; p < 0.001) compared to SD-rats. HFD-rats exhibited early stages of ORG, with predominant mesangial matrix increase and podocyte hypertrophy (PH). These lesions correlated with differentially expressed (DE) genes and miRNAs. Functional analysis showed that miR-205, which was DE in both the kidneys and urine of HFD-rats, negatively regulated the PTEN gene, promoting lipid endocytosis in podocytes. The downregulation of PTEN was proved through a higher PTEN/nephrin ratio in the SD-rats and the presence of lipid vacuoles in HFD-podocytes. This study has found a specific targetome of miRNAs and gene expression in early stages of ORG. Also, it emphasizes the potential value of miR-205 as a urinary biomarker for detecting podocyte injury in ORG, offering a tool for early diagnosis, and opening new avenues for future therapeutic research of obesity-related glomerulopathy.
dc.description.abstract
This research was funded by Instituto de Salud Carlos III, grant number PI18-01951.
dc.format
application/pdf
dc.relation
International Journal of Molecular Sciences;25(12)
dc.relation
https://doi.org/10.3390/ijms25126437
dc.rights
Attribution 4.0 International
dc.rights
http://creativecommons.org/licenses/by/4.0/
dc.rights
info:eu-repo/semantics/openAccess
dc.subject
Ronyons - Malalties - Aspectes genètics
dc.subject
DISEASES::Male Urogenital Diseases::Urologic Diseases::Kidney Diseases
dc.subject
Other subheadings::Other subheadings::Other subheadings::/genetics
dc.subject
PHENOMENA AND PROCESSES::Physiological Phenomena::Body Constitution::Body Weights and Measures::Body Size::Body Weight::Overweight::Obesity
dc.subject
PHENOMENA AND PROCESSES::Physiological Phenomena::Diet, Food, and Nutrition::Nutritional Physiological Phenomena::Diet::Diet, High-Fat
dc.subject
CHEMICALS AND DRUGS::Nucleic Acids, Nucleotides, and Nucleosides::Antisense Elements (Genetics)::RNA, Antisense::MicroRNAs
dc.subject
ENFERMEDADES::enfermedades urogenitales masculinas::enfermedades urológicas::enfermedades renales
dc.subject
Otros calificadores::Otros calificadores::Otros calificadores::/genética
dc.subject
FENÓMENOS Y PROCESOS::fenómenos fisiológicos::constitución corporal::pesos y medidas corporales::tamaño corporal::peso corporal::sobrepeso::obesidad
dc.subject
FENÓMENOS Y PROCESOS::fenómenos fisiológicos::dieta, alimentación y nutrición::fenómenos fisiológicos nutricionales::dieta::alimentación rica en grasa
dc.subject
COMPUESTOS QUÍMICOS Y DROGAS::nucleótidos y nucleósidos de ácidos nucleicos::elementos antisentido (genética)::ARN antiparalelo::microARN
dc.title
Integrated miRNA–mRNA Analysis Reveals Critical miRNAs and Targets in Diet-Induced Obesity-Related Glomerulopathy
dc.type
info:eu-repo/semantics/article
dc.type
info:eu-repo/semantics/publishedVersion
