dc.contributor
Institut Català de la Salut
dc.contributor
[Polonio-Alcalá E, Rabionet M] Product, Process and Production Engineering Research Group (GREP), Department of Mechanical Engineering and Industrial Construction, University of Girona, Girona, Spain. New Therapeutic Targets Laboratory (TargetsLab)-Oncology Unit, Department of Medical Sciences, Faculty of Medicine, University of Girona, Girona, Spain. [Ruiz-Martínez S, Palomeras S, Puig T] New Therapeutic Targets Laboratory (TargetsLab)-Oncology Unit, Department of Medical Sciences, Faculty of Medicine, University of Girona, Girona, Spain. [Porta R] New Therapeutic Targets Laboratory (TargetsLab)-Oncology Unit, Department of Medical Sciences, Faculty of Medicine, University of Girona, Girona, Spain. Medical Oncology Department, Catalan Institute of Oncology, Girona, Spain. [Vásquez-Dongo C] New Therapeutic Targets Laboratory (TargetsLab)-Oncology Unit, Department of Medical Sciences, Faculty of Medicine, University of Girona, Girona, Spain. Department of Pathology, Hospital Universitari de Girona Doctor Josep Trueta, Institut Català de la Salut (ICS), Girona, Spain. [Bosch-Barrera J] Medical Oncology Department, Catalan Institute of Oncology, Girona, Spain. [ Ciurana J] Product, Process and Production Engineering Research Group (GREP), Department of Mechanical Engineering and Industrial Construction, University of Girona, Girona, Spain
dc.contributor
Hospital Universitari de Girona Dr Josep Trueta
dc.contributor.author
Polonio-Alcalá, Emma
dc.contributor.author
Rabionet Diaz, Marc
dc.contributor.author
Ruiz-Martínez, Santiago
dc.contributor.author
Palomeras Pairet, Sònia
dc.contributor.author
PORTA, RUT
dc.contributor.author
Vasquez-Dongo, Carmen Amalia
dc.contributor.author
Puig, Teresa
dc.contributor.author
Ciurana, Joaquim
dc.contributor.author
Bosch-Barrera, Joaquim
dc.date.accessioned
2025-10-24T07:47:04Z
dc.date.available
2025-10-24T07:47:04Z
dc.date.issued
2024-04-02T10:10:57Z
dc.date.issued
2024-04-02T10:10:57Z
dc.date.issued
2021-10-22
dc.identifier
Polonio-Alcalá E, Rabionet M, Ruiz-Martinez S, Palomeras S, Porta R, Vásquez-Dongo C, et al. Polycaprolactone Electrospun Scaffolds Produce an Enrichment of Lung Cancer Stem Cells in Sensitive and Resistant EGFRm Lung Adenocarcinoma. Cancers (Basel). 2021 Oct 22;13(21):5320.
dc.identifier
https://hdl.handle.net/11351/11265
dc.identifier
10.3390/cancers13215320
dc.identifier.uri
https://hdl.handle.net/11351/11265
dc.description.abstract
Cultiu cel·lular tridimensional; Cèl·lules mare; Càncer de pulmó
dc.description.abstract
Cultivo celular tridimensional; Células madre; Cáncer de pulmón
dc.description.abstract
Three Dimensional Cell Culture; Stem Cell; Lung neoplasm
dc.description.abstract
The establishment of a three-dimensional (3D) cell culture model for lung cancer stem cells (LCSCs) is needed because the study of these stem cells is unable to be done using flat surfaces. The study of LCSCs is fundamental due to their key role in drug resistance, tumor recurrence, and metastasis. Hence, the purpose of this work is the evaluation of polycaprolactone electrospun (PCL-ES) scaffolds for culturing LCSCs in sensitive and resistant EGFR-mutated (EGFRm) lung adenocarcinoma cell models. We performed a thermal, physical, and biological characterization of 10% and 15%-PCL-ES structures. Several genes and proteins associated with LCSC features were analyzed by RT-qPCR and Western blot. Vimentin and CD133 tumor expression were evaluated in samples from 36 patients with EGFRm non-small cell lung cancer through immunohistochemistry. Our findings revealed that PC9 and PC9-GR3 models cultured on PCL-ES scaffolds showed higher resistance to osimertinib, upregulation of ABCB1, Vimentin, Snail, Twist, Sox2, Oct-4, and CD166, downregulation of E-cadherin and CD133, and the activation of Hedgehog pathway. Additionally, we determined that the non-expression of CD133 was significantly associated with a low degree of histological differentiation, disease progression, and distant metastasis. To sum up, we confirmed PCL-ES scaffolds as a suitable 3D cell culture model for the study of the LCSC niche.
dc.description.abstract
This research was funded by Fundación Ramón Areces, Instituto de Salud Carlos III (PI1900372), Ministerio de Economía y Competitividad (DPI2016- 77156-R), and AstraZeneca
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dc.relation
Cancers;13(21)
dc.relation
https://doi.org/10.3390/cancers13215320
dc.rights
Attribution 4.0 International
dc.rights
http://creativecommons.org/licenses/by/4.0/
dc.rights
info:eu-repo/semantics/openAccess
dc.subject
Cèl·lules mare
dc.subject
Pulmons--Càncer
dc.subject
Pulmons - Càncer
dc.subject
ANATOMY::Cells::Stem Cells::Neoplastic Stem Cells
dc.subject
ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Cytological Techniques::Cell Culture Techniques
dc.subject
DISEASES::Neoplasms::Neoplasms by Site::Thoracic Neoplasms::Respiratory Tract Neoplasms::Lung Neoplasms
dc.subject
TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::diagnóstico::técnicas y procedimientos diagnósticos::técnicas de laboratorio clínico::técnicas citológicas::técnicas de cultivo celular
dc.subject
ENFERMEDADES::neoplasias::neoplasias por localización::neoplasias torácicas::neoplasias del tracto respiratorio::neoplasias pulmonares
dc.title
Polycaprolactone Electrospun Scaffolds Produce an Enrichment of Lung Cancer Stem Cells in Sensitive and Resistant EGFRm Lung Adenocarcinoma
dc.type
info:eu-repo/semantics/article
