dc.contributor
[Villar-Hernández R] Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Institut d'Investigació Germans Trias i Pujol, Barcelona, Spain. CIBER Enfermedades Respiratorias, CIBERES, Instituto de Salud Carlos III, Madrid, Spain. Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Barcelona, Spain. R&D Department, Genome Identification Diagnostics (GenID) GmbH, Strassberg, Germany. [Latorre I] Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Institut d'Investigació Germans Trias i Pujol, Barcelona, Spain. CIBER Enfermedades Respiratorias, CIBERES, Instituto de Salud Carlos III, Madrid, Spain. Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Barcelona, Spain. [Noguera-Julian A] Malalties Infeccioses i Resposta Inflamatòria Sistèmica en Pediatria, Servei de Malalties Infeccioses i Patologia Importada, Institut de Recerca Sant Joan de Déu, Barcelona, Spain. Red de Investigación Translacional en Infectología Pediátrica, RITIP, Madrid, Spain. Departament de Cirurgia i Especialitats Medicoquirúrgiques, Universitat de Barcelona, Barcelona, Spain. CIBER de Epidemiología y Salud Pública, CIBERESP, Instituto de Salud Carlos III, Madrid, Spain. [Martínez-Planas A] Malalties Infeccioses i Resposta Inflamatòria Sistèmica en Pediatria, Servei de Malalties Infeccioses i Patologia Importada, Institut de Recerca Sant Joan de Déu, Barcelona, Spain. Red de Investigación Translacional en Infectología Pediátrica, RITIP, Madrid, Spain. [Minguell L, Vallmanya T] Department of Pediatrics, Hospital Universitari Arnau de Vilanova, Lleida, Spain. [Galea Y] Servei de Pneumologia, Hospital General de Granollers, Granollers, Spain
dc.contributor
Hospital General de Granollers
dc.contributor.author
Villar Hernández, Raquel
dc.contributor.author
Latorre, Irene
dc.contributor.author
Noguera-Julian, Antoni
dc.contributor.author
martinez planas, aina
dc.contributor.author
Minguell, Laura
dc.contributor.author
Vallmanya, Teresa
dc.contributor.author
Galea, Yolanda
dc.date.accessioned
2025-10-24T10:37:26Z
dc.date.available
2025-10-24T10:37:26Z
dc.date.issued
2024-01-26T14:04:40Z
dc.date.issued
2024-01-26T14:04:40Z
dc.date.issued
2023-12-18
dc.identifier
Villar-Hernández R, Latorre I, Noguera-Julian A, Martínez-Planas A, Minguell L, Vallmanya T, et al. Development and Evaluation of an NTM-IGRA to Guide Pediatric Lymphadenitis Diagnosis. Pediatr Infect Dis J. 2023 Dec 18.
dc.identifier
https://hdl.handle.net/11351/10918
dc.identifier
10.1097/INF.0000000000004211
dc.identifier.uri
https://hdl.handle.net/11351/10918
dc.description.abstract
Nontuberculous mycobacteria; Tuberculosis; Glycopeptidolipids
dc.description.abstract
Micobacterias no tuberculosas; Tuberculosis; Glicopeptidolípidos
dc.description.abstract
Micobacteris no tuberculosos; Tuberculosi; Glicopeptidolípids
dc.description.abstract
Background: Diagnosis of nontuberculous mycobacteria (NTM) infections remains a challenge. In this study, we describe the evaluation of an immunological NTM-interferon (IFN)-γ release assay (IGRA) that we developed using glycopeptidolipids (GPLs) as NTM-specific antigens.
Methods: We tested the NTM-IGRA in 99 samples from pediatric patients. Seventy-five were patients with lymphadenitis: 25 were NTM confirmed, 45 were of unknown etiology but compatible with mycobacterial infection and 5 had lymphadenitis caused by an etiologic agent other than NTM. The remaining 24 samples were from control individuals without lymphadenitis (latently infected with M. tuberculosis, uninfected controls and active tuberculosis patients). Peripheral blood mononuclear cells were stimulated overnight with GPLs. Detection of IFN-γ producing cells was evaluated by enzyme-linked immunospot assay.
Results: NTM culture-confirmed lymphadenitis patient samples had a significantly higher response to GPLs than the patients with lymphadenitis of unknown etiology but compatible with mycobacterial infection (P < 0.001) and lymphadenitis not caused by NTM (P < 0.01). We analyzed the response against GPLs in samples from unknown etiology lymphadenitis but compatible with mycobacterial infection cases according to the tuberculin skin test (TST) response, and although not statistically significant, those with a TST ≥5 mm had a higher response to GPLs when compared with the TST <5 mm group.
Conclusions: Stimulation with GPLs yielded promising results in detecting NTM infection in pediatric patients with lymphadenitis. Our results indicate that the test could be useful to guide the diagnosis of pediatric lymphadenitis. This new NTM-IGRA could improve the clinical handling of NTM-infected patients and avoid unnecessary misdiagnosis and treatments.
dc.format
application/pdf
dc.publisher
Lippincott, Williams & Wilkins
dc.relation
The Pediatric Infectious Disease Journal;
dc.relation
https://doi.org/10.1097/INF.0000000000004211
dc.rights
Attribution-NonCommercial-NoDerivatives 4.0 International
dc.rights
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights
info:eu-repo/semantics/openAccess
dc.subject
Micobacteriosis
dc.subject
ORGANISMS::Bacteria::Gram-Positive Bacteria::Actinobacteria::Actinomycetales::Mycobacteriaceae::Mycobacterium::Nontuberculous Mycobacteria
dc.subject
CHEMICALS AND DRUGS::Carbohydrates::Glycoconjugates::Glycopeptides
dc.subject
DISEASES::Hemic and Lymphatic Diseases::Lymphatic Diseases::Lymphadenitis
dc.subject
ORGANISMOS::Bacteria::bacterias grampositivas::Actinobacteria::Actinomycetales::Mycobacteriaceae::Mycobacterium::micobacterias no tuberculosas
dc.subject
COMPUESTOS QUÍMICOS Y DROGAS::hidratos de carbono::glicoconjugados::glicopéptidos
dc.subject
ENFERMEDADES::enfermedades hematológicas y linfáticas::enfermedades linfáticas::linfadenitis
dc.title
Development and Evaluation of an NTM-IGRA to Guide Pediatric Lymphadenitis Diagnosis
dc.type
info:eu-repo/semantics/article
dc.type
info:eu-repo/semantics/acceptedVersion
