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Autor/a:
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Moreno, R.; Rojas, L.A.; Vilardell, Felip; Cervera Soriano, Vanessa; García-Castro, J.; Fajardo, C. A.; Alemany, R.
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Notas:
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Antitumor efficacy of systemically administered oncolytic adenoviruses (OAdv) is limited due to diverse factors such as liver sequestration, neutralizing interactions in blood, elimination by the immune system, and physical barriers in tumors. It is therefore of clinical relevance to improve OAdv bioavailability and tumor delivery. Among the variety of tumor-targeting strategies, the use of stem cells and specifically bone marrow-derived mesenchymal stem cells (BM-MSCs) is of particular interest due to their tumor tropism and immunomodulatory properties. Nonetheless, the invasive methods to obtain these cells, the low number of MSCs present in the bone marrow, and their restricted in vitro expansion represent major obstacles for their use in cancer treatments, pointing out the necessity to identify an alternative source of MSCs. Here, we have evaluated the use of menstrual blood-derived mesenchymal stem cells (MenSCs) as cell carriers for regional delivery of an OAdv in the tumor. Our results indicate that MenSCs can be isolated without invasive methods, they have an increased proliferation rate compared to BM-MSCs, and they can be efficiently infected with different serotype 5-based capsid-modified adenoviruses, leading to viral replication and release. In addition, our in vivo studies confirmed the tumor-homing properties of MenSCs after regional administration.
This work was supported by Asociación Española Contra el Cáncer (AECC), BIO2014-57716-C2-1-R grant and PI14CIII/00005 to J G-C from the Ministerio de Economía y Competitividad of Spain, Adenonet BIO2015-68990- REDT from the Ministerio de Economía y Competitividad of Spain, Red ADVANCE (CAT) Project COMRDI15-1-0013 from Ris3CAT, and 2014SGR364 research grant from the “Generalitat de Catalunya,” cofunded by the European Regional Development Fund, a way to Build Europe |
