dc.contributor.author |
Mota Martorell, Natalia |
dc.contributor.author |
Jové Font, Mariona |
dc.contributor.author |
Pradas Barriga, Irene |
dc.contributor.author |
Sanchez, Isabel |
dc.contributor.author |
Gómez, José |
dc.contributor.author |
Naudí i Farré, Alba |
dc.contributor.author |
Barja, Gustavo |
dc.contributor.author |
Pamplona Gras, Reinald |
dc.date |
2021-03-09T07:24:13Z |
dc.date |
2021-03-09T07:24:13Z |
dc.date |
2020-07-01 |
dc.date |
2021-03-09T07:24:13Z |
dc.identifier |
2213-2317 |
dc.identifier |
http://hdl.handle.net/10459.1/70698 |
dc.identifier |
https://doi.org/10.1016/j.redox.2020.101539 |
dc.identifier.uri |
http://hdl.handle.net/10459.1/70698 |
dc.description |
Mitochondrial reactive oxygen species (ROS) production, specifically at complex I (Cx I), has been widely suggested to be one of the determinants of species longevity. The present study follows a comparative approach to analyse complex I in heart tissue from 8 mammalian species with a longevity ranging from 3.5 to 46 years. Gene expression and protein content of selected Cx I subunits were analysed using droplet digital PCR (ddPCR) and western blot, respectively. Our results demonstrate: 1) the existence of species-specific differences in gene expression and protein content of Cx I in relation to longevity; 2) the achievement of a longevity phenotype is associated with low protein abundance of subunits NDUFV2 and NDUFS4 from the matrix hydrophilic domain of Cx I; and 3) long-lived mammals show also lower levels of VDAC (voltage-dependent anion channel) amount. These differences could be associated with the lower mitochondrial ROS production and slower aging rate of long-lived animals and, unexpectedly, with a low content of the mitochondrial permeability transition pore in these species. |
dc.description |
This work was supported by the Spanish Ministry of Economy and Competitiveness, Institute of Health Carlos III (grant number PI14/00328), the Spanish Ministry of Science, Innovation and Universities (RTI2018-099200-B-I00), and the Generalitat of Catalonia, Agency for Management of University and Research Grants (2017SGR696) and Department of Health (SLT002/16/00250) to R.P, and PR [19] BIO MET 0155 to GB.This study has been co-financed by FEDER funds from the European Union (“A way to build Europe”). IRBLleida is a CERCA Programme/Generalitat of Catalonia. |
dc.format |
application/pdf |
dc.publisher |
Elsevier |
dc.relation |
MINECO/PN2017-2020/RTI2018-099200-B-I00 |
dc.relation |
Reproducció del document publicat a: https://doi.org/10.1016/j.redox.2020.101539 |
dc.relation |
Redox Biology, 2020, vol. 34, núm. 101539, p. 1-10 |
dc.rights |
cc-by-nc-nd (c) Mota Martorell et al., 2020 |
dc.rights |
http://creativecommons.org/licenses/by-nc-nd/3.0/es |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
Complex I |
dc.subject |
Droplet digital PCR |
dc.subject |
Longevity |
dc.subject |
Mammals |
dc.subject |
Mitochondria |
dc.subject |
NDUFV2 subunit |
dc.subject |
NDUFS4 subunit |
dc.subject |
VDAC |
dc.subject |
Western blot |
dc.title |
Low abundance of NDUFV2 and NDUFS4 subunits of the hydrophilic complex I domain and VDAC1 predicts mammalian longevity |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/publishedVersion |