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Title:
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Constitutive expression of a barley Fe phytosiderophore transporter increases alkaline soil tolerance and results in iron partitioning between vegetative and storage tissues under stress
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Author:
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Gómez Galera, Sonia; Sudhakar, Duraialagaraja; Pelacho Aja, Ana Mª; Capell Capell, Teresa; Christou, Paul
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Notes:
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Cereals have evolved chelation systems to mobilize insoluble iron in the soil, but in rice this process is
rather inefficient, making the crop highly susceptible to alkaline soils. We therefore engineered rice to
express the barley iron-phytosiderophore transporter (HvYS1), which enables barley plants to take up
iron from alkaline soils. A representative transgenic rice line was grown in standard (pH 5.5) or alkaline
soil (pH 8.5) to evaluate alkaline tolerance and iron mobilization. Transgenic plants developed secondary
tillers and set seeds when grown in standard soil although iron concentration remained similar in leaves
and seeds compared to wild type. However, when grown in alkaline soil transgenic plants exhibited
enhanced growth, yield and iron concentration in leaves compared to the wild type plants which were
severely stunted. Transgenic plants took up iron more efficiently from alkaline soil compared to wild
type, indicating an enhanced capacity to increase iron mobility ex situ. Interestingly, all the additional
iron accumulated in vegetative tissues, i.e. there was no difference in iron concentration in the seeds of
wild type and transgenic plants. Our data suggest that iron uptake from the rhizosphere can be enhanced
through expression of HvYS1 and confirm the operation of a partitioning mechanism that diverts iron to
leaves rather than seeds, under stress.
P.C. acknowledges the financial support of the Spanish Ministry of Science and Innovation (grant number BFU2007-61413) and the European Research Council for the BIOFORCE advanced grant. |
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Subject(s):
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-Transgenic rice
-Iron uptake
-Alkalinity
-Tolerance |
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Rights:
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(c) Elsevier Masson SAS, 2012
info:eu-repo/semantics/restrictedAccess |
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Document type:
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article
publishedVersion |
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Published by:
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Elsevier
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