dc.contributor.author
Yeste Oliveras, Marc
dc.contributor.author
Estrada Paqui, Efrén
dc.contributor.author
Casas Roqueta, Isabel
dc.contributor.author
Bonet, Sergi
dc.contributor.author
Rodríguez-Gil, Joan E
dc.date.accessioned
2025-02-04T14:10:43Z
dc.date.available
2025-02-04T14:10:43Z
dc.date.issued
2013-04-01
dc.identifier
http://hdl.handle.net/10256/26283
dc.identifier.uri
https://hdl.handle.net/10256/26283
dc.description.abstract
The main aim of the present study was to determine whether differences in the amounts of free cysteine residues in sperm nucleoproteins, which are a direct marker of the integrity of the disulfide bonds between nucleoproteins, existed between good (GFE) and poor boar freezability ejaculates (PFE) during the different steps of the freeze-thawing process. The analyzed steps were: (1) immediately before starting cryopreservation (17 °C), (2) at the end of the cooling step (5 °C), and (3) 30, and (4) 240 minutes after thawing. In addition, the present study also sought to determine whether GFE and PFE differed in the amounts of peroxides and superoxides generated during freeze-thawing as an overall measure of the boar sperm reactive oxygen species (ROS) accumulation rate. According to our results, PFE present lower resistance than GFE to cryopreservation-induced alterations of disulfide bonds between nucleoproteins, because levels of cysteine free residues were higher in PFE than in GFE at 30 and 240 minutes after thawing. On the other hand, no significant differences were observed between GFE and PFE in ROS levels during freeze-thawing. In conclusion, PFE are less resistant than GFE to cryopreservation not only in terms of sperm motility and membrane integrity, but also in the integrity of nucleoprotein structure. However, this difference between PFE and GFE in the resistance of the nucleoprotein structure to freeze-thawing is not linked with concomitant changes in ROS levels
dc.format
application/pdf
dc.relation
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.theriogenology.2013.01.008
dc.relation
info:eu-repo/semantics/altIdentifier/issn/0093-691X
dc.relation
info:eu-repo/semantics/altIdentifier/eissn/1879-3231
dc.rights
Reconeixement-NoComercial-SenseObraDerivada 4.0 Internacional
dc.rights
http://creativecommons.org/licenses/by-nc-nd/4.0
dc.rights
info:eu-repo/semantics/openAccess
dc.source
© Theriogenology, 2013, vol. 79, núm. 6, p. 929-939
dc.source
Articles publicats (D-B)
dc.source
Yeste Oliveras, Marc Estrada Paqui, Efrén Casas Roqueta, Isabel Bonet, Sergi Rodríguez-Gil, Joan E 2013 Good and bad freezability boar ejaculates differ in the integrity of nucleoprotein structure after freeze-thawing but not in ROS levels Theriogenology 79 6 929 939
dc.subject
Estrès oxidatiu
dc.subject
Oxidative stress
dc.subject
Semen -- Crioconservació
dc.subject
Semen -- Cryopreservation
dc.subject
Porcs -- Espermatozoides -- Investigació
dc.subject
Swine -- Spermatozoa -- Research
dc.subject
Porcs -- Espermatozoides -- Criopreservació
dc.subject
Swine -- Spermatozoa -- Cryopreservation
dc.title
Good and bad freezability boar ejaculates differ in the integrity of nucleoprotein structure after freeze-thawing but not in ROS levels
dc.type
info:eu-repo/semantics/article
dc.type
info:eu-repo/semantics/acceptedVersion
