Título:
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Evidence for functional pre-coupled complexes of receptor heteromers and adenylyl cyclase
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Autor/a:
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Navarro Brugal, Gemma; Cordomí, Arnau; Casadó Anguera, Verónica; Moreno Guillén, Estefanía; Cai, Ning-Sheng; Cortes, Antoni; Canela Campos, Enric I.; Dessauer, Carmen W.; Casadó, Vicent; Pardo, Leonardo; Lluís i Biset, Carme; Ferré, Sergi
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Otros autores:
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Universitat de Barcelona |
Abstract:
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G protein-coupled receptors (GPCRs), G proteins and adenylyl cyclase (AC) comprise one of the most studied transmembrane cell signaling pathways. However, it is unknown whether the ligand-dependent interactions between these signaling molecules are based on random collisions or the rearrangement of pre-coupled elements in a macromolecular complex. Furthermore, it remains controversial whether a GPCR homodimer coupled to a single heterotrimeric G protein constitutes a common functional unit. Using a peptide-based approach, we here report evidence for the existence of functional pre-coupled complexes of heteromers of adenosine A2A receptor and dopamine D2 receptor homodimers coupled to their cognate Gs and Gi proteins and to subtype 5 AC. We also demonstrate that this macromolecular complex provides the necessary frame for the canonical Gs-Gi interactions at the AC level, sustaining the ability of a Gi-coupled GPCR to counteract AC activation mediated by a Gs-coupled GPCR. |
Materia(s):
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-Adenosina -Receptors cel·lulars -Adenosine -Cell receptors |
Derechos:
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cc-by (c) Navarro Brugal, Gemma et al., 2018
http://creativecommons.org/licenses/by/3.0/es |
Tipo de documento:
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Artículo Artículo - Versión publicada |
Editor:
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Nature Publishing Group
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Compartir:
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