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Título:	Coaggregation, cointernalization, and codesensitization of adenosine A2A receptors and dopamine D2 receptors
Autor/a:	Hillion, Joëlle; Canals, Meritxell; Torvinen, Maria; Casadó, Vicent; Scott, Rizaldy; Terasmaa, Anton; Hansson, Anita; Watson, Stanley; Olah, Mark E.; Mallol Montero, Josefa; Canela Campos, Enric I.; Zoli, Michele; Agnati, Luigi F.; Ibanez, Carlos F.; Lluís i Biset, Carme; Franco Fernández, Rafael; Ferré, Sergi; Fuxe, Kjell
Otros autores:	Universitat de Barcelona
Abstract:	Antagonistic and reciprocal interactions are known to exist between adenosine and dopamine receptors in the striatum. In the present study, double immunofluorescence experiments with confocal laser microscopy showed a high degree of colocalization of adenosine A(2A) receptors (A(2A)R) and dopamine D(2) receptors (D(2)R) in cell membranes of SH-SY5Y human neuroblastoma cells stably transfected with human D(2)R and in cultured striatal cells. A(2A)R/D(2)R heteromeric complexes were demonstrated in coimmunoprecipitation experiments in membrane preparations from D(2)R-transfected SH-SY5Y cells and from mouse fibroblast Ltk(-) cells stably transfected with human D(2)R (long form) and transiently cotransfected with the A(2A)R double-tagged with hemagglutinin. Long term exposure to A(2A)R and D(2)R agonists in D(2)R-cotransfected SH-SY5Y cells resulted in coaggregation, cointernalization and codesensitization of A(2A)R and D(2)R. These results give a molecular basis for adenosine-dopamine antagonism at the membrane level and have implications for treatment of Parkinson's disease and schizophrenia, in which D(2)R are involved.
Materia(s):	-Receptors cel·lulars -Malalties del sistema nerviós -Adenosina -Cell receptors -Nervous system diseases -Adenosine
Derechos:	(c) American Society for Biochemistry and Molecular Biology, 2002
Tipo de documento:	Artículo Artículo - Versión publicada
Editor:	American Society for Biochemistry and Molecular Biology
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