Nutrient composition promotes switching between pellicle and bottom biofilm in Salmonella

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RECERCAT Home > Universitat de Barcelona > Genètica, Microbiologia i Estadística > Articles publicats en revistes (Genètica, Microbiologia i Estadística) > View document

To access the full text documents, please follow this link: http://hdl.handle.net/2445/117751

dc.contributor	Universitat de Barcelona
dc.contributor.author	Paytubi Casabona, Sònia
dc.contributor.author	Cansado, Cintia
dc.contributor.author	Madrid Xufré, Cristina
dc.contributor.author	Balsalobre Parra, Carlos
dc.date	2017-11-14T16:03:10Z
dc.date	2017-11-14T16:03:10Z
dc.date	2017-11-07
dc.date	2017-11-14T16:03:10Z
dc.identifier.citation	1664-302X
dc.identifier.citation	674243
dc.identifier.uri	http://hdl.handle.net/2445/117751
dc.description.abstract	Salmonella is one of the most frequently reported causes of foodborne illness worldwide. Non-typhoidal serovars cause gastroenteritis in humans. Salmonella can grow on surfaces forming biofilms, contributing to its persistence since biofilms are difficult to eradicate due to the high resistance to antimicrobials and disinfectants. It has been described that there are two crucial biofilm promoting factors in Salmonella: curli and cellulose. The expression of both factors is coordinately regulated by the transcriptional regulator CsgD. Most biofilm studies of Salmonella have been performed by growing bacteria in low osmolarity rich medium and low temperature (25°C). In such conditions, the biofilm is formed at the air-liquid interface (pellicle biofilm). Remarkably, when Salmonella grow in minimal medium, biofilm formation switches from the air-liquid interface to the solid-liquid interface (bottom biofilm). In this report, the switching between pellicle and bottom biofilm has been characterized. Our data indicate that curli, but not cellulose, is crucial for the formation of both kinds of biofilms. In minimal medium, conditions promoting formation of bottom biofilm, a high transcriptional expression of csgD and consequently of the genes involved in the synthesis of curli and cellulose was detected. The nutritional status of the cells seems to be pivotal for the spatial distribution of the biofilms formed. When bacteria is growing in minimal medium the addition of amino acids downregulates the expression of csgB and causes the switch between bottom and pellicle biofilm. The crosstalk between general metabolism and biofilm formation is also highlighted by the fact that the metabolic sensor cAMP modulates the type of biofilm generated by Salmonella. Moreover, cAMP regulates transcriptional expression of csgD and stimulates pellicle biofilm formation, suggesting that the physiological conditions define the type of biofilm formed by Salmonella. The consequences of the switching between pellicle and bottom biofilm during either infection or survival in natural environments remain undercover.
dc.format	12 p.
dc.format	application/pdf
dc.language.iso	eng
dc.publisher	Frontiers Media
dc.relation	Reproducció del document publicat a: https://doi.org/10.3389/fmicb.2017.02160
dc.relation	Frontiers in Microbiology, 2017, vol. 8, p. 2160
dc.relation	https://doi.org/10.3389/fmicb.2017.02160
dc.rights	cc-by (c) Paytubi, Sonia et al., 2017
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	http://creativecommons.org/licenses/by/3.0/es
dc.subject	Biofilms
dc.subject	Salmonel·la
dc.subject	Biofilms
dc.subject	Salmonella
dc.title	Nutrient composition promotes switching between pellicle and bottom biofilm in Salmonella
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion

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