Título:
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Molecular surveillance of pfhrp2 and pfhrp3 deletions in
Plasmodium falciparum isolates from Mozambique
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Autor/a:
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Gupta, Himanshu; Matambisso, Gloria; Galatas, Beatriz; Cisteró, Pau; Nhamussua, Lidia; Simone, Wilson; Cunningham, Jane; Rabinovich, Regina; Alonso, Pedro; Saute, Francisco; Aide, Pedro Carlos Paulino; Mayor Aparicio, Alfredo Gabriel
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Abstract:
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BACKGROUND: Malaria programmes use Plasmodium falciparum
histidine-rich protein-2 (PfHRP2) based rapid diagnostic tests
(RDTs) for malaria diagnosis. The deletion of this target
antigen could potentially lead to misdiagnosis, delayed
treatment and continuation of active transmission. METHODS:
Plasmodium falciparum isolates (n = 1162) collected in Southern
Mozambique were assessed by RDTs, microscopy and/or 18SrRNA
qPCR. pfhrp2 and pfhrp3 deletions were investigated in isolates
from individuals who were negative by RDT but positive by
microscopy and/or qPCR (n = 69) using gene-specific PCRs, with
kelch13 PCR as the parasite DNA control. RESULTS: Lack of pfhrp2
PCR amplification was observed in one of the 69 isolates
subjected to molecular analysis [1.45% (95% CI 0.3-7.8%)].
CONCLUSIONS: The low prevalence of pfhrp2 deletions suggests
that RDTs will detect the vast majority of the P. falciparum
infections. Nevertheless, active surveillance for changing
deletion frequencies is required. |
Materia(s):
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-Malària -Moçambic -Malaria -Mozambique |
Derechos:
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cc by (c) Gupta et al., 2017
http://creativecommons.org/licenses/by/4.0/ |
Tipo de documento:
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Artículo Artículo - Versión publicada |
Editor:
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BioMed Central
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