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| dc.contributor | Universitat de Barcelona |
|---|---|
| dc.contributor.author | Guillaumet Adkins, Amy |
| dc.contributor.author | Rodríguez Esteban, Gustavo |
| dc.contributor.author | Mereu, Elisabetta |
| dc.contributor.author | Méndez Lago, María |
| dc.contributor.author | Jaitin, Diego A. |
| dc.contributor.author | Villanueva Garatachea, Alberto |
| dc.contributor.author | Vidal-Bel, August |
| dc.contributor.author | Martínez Martí, Aleix |
| dc.contributor.author | Felip, Enriqueta |
| dc.contributor.author | Vivancos, Ana |
| dc.contributor.author | Keren-Shaul, Hadas |
| dc.contributor.author | Heath, Simon |
| dc.contributor.author | Gut, Marta |
| dc.contributor.author | Amit, Ido |
| dc.contributor.author | Gut, Ivo |
| dc.contributor.author | Heyn, Holger |
| dc.date | 2017-06-12T10:37:35Z |
| dc.date | 2017-06-12T10:37:35Z |
| dc.date | 2017-03-01 |
| dc.date | 2017-06-12T10:37:35Z |
| dc.identifier.citation | 1474-7596 |
| dc.identifier.citation | 669574 |
| dc.identifier.uri | http://hdl.handle.net/2445/112225 |
| dc.description.abstract | A variety of single-cell RNA preparation procedures have been described. So far, protocols require fresh material, which hinders complex study designs. We describe a sample preservation method that maintains transcripts in viable single cells, allowing one to disconnect time and place of sampling from subsequent processing steps. We sequence single-cell transcriptomes from >1000 fresh and cryopreserved cells using 3'-end and full-length RNA preparation methods. Our results confirm that the conservation process did not alter transcriptional profiles. This substantially broadens the scope of applications in single-cell transcriptomics and could lead to a paradigm shift in future study designs. |
| dc.format | 15 p. |
| dc.format | application/pdf |
| dc.language.iso | eng |
| dc.publisher | BioMed Central |
| dc.relation | Reproducció del document publicat a: https://doi.org/10.1186/s13059-017-1171-9 |
| dc.relation | Genome Biology, 2017, vol. 18, p. 45 |
| dc.relation | https://doi.org/10.1186/s13059-017-1171-9 |
| dc.rights | cc-by (c) Guillaumet-Adkins, Amy et al., 2017 |
| dc.rights | info:eu-repo/semantics/openAccess |
| dc.rights | http://creativecommons.org/licenses/by/3.0/es |
| dc.subject | RNA |
| dc.subject | Crioconservació d'òrgans, teixits, etc. |
| dc.subject | Criobiologia |
| dc.subject | Transcripció genètica |
| dc.subject | Genòmica |
| dc.subject | Cèl·lules |
| dc.subject | RNA |
| dc.subject | Cryopreservation of organs, tissues, etc. |
| dc.subject | Cryobiology |
| dc.subject | Genetic transcription |
| dc.subject | Genomics |
| dc.subject | Cells |
| dc.title | Single-cell transcriptome conservation in cryopreserved cells and tissues |
| dc.type | info:eu-repo/semantics/article |
| dc.type | info:eu-repo/semantics/publishedVersion |
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