dc.contributor.author |
Gomes, Cláudia |
dc.contributor.author |
Palma, Noemí |
dc.contributor.author |
Pons, Maria J. |
dc.contributor.author |
Magallón Tejada, Ariel |
dc.contributor.author |
Sandoval, Isabel |
dc.contributor.author |
Tinco-Valdez, Carmen |
dc.contributor.author |
Gutarra, Carlos |
dc.contributor.author |
Valle Mendoza, Juana del |
dc.contributor.author |
Ruiz, Joaquim |
dc.contributor.author |
Matsuoka, Mayumi |
dc.date |
2016-10-07T14:06:20Z |
dc.date |
2016-10-07T14:06:20Z |
dc.date |
2016-09-14 |
dc.date |
2016-10-05T18:00:57Z |
dc.identifier.citation |
1935-2727 |
dc.identifier.uri |
http://hdl.handle.net/2445/102466 |
dc.format |
17 p. |
dc.format |
application/pdf |
dc.language.iso |
eng |
dc.publisher |
Public Library of Science (PLoS) |
dc.relation |
Reproducció del document publicat a:
http://dx.doi.org/10.1371/journal.pntd.0004989 |
dc.relation |
PLoS Neglected Tropical Diseases, 2016, vol. 10, num. 9, p.
e0004989 |
dc.relation |
http://dx.doi.org/10.1371/journal.pntd.0004989 |
dc.rights |
cc by (c) Gomes et al., 2016 |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.rights |
http://creativecommons.org/licenses/by/3.0/es/ |
dc.subject |
Malalties bacterianes |
dc.subject |
Paisatge rural |
dc.subject |
Antígens bacterians |
dc.subject |
Bacterial diseases |
dc.subject |
Rural landscape |
dc.subject |
Bacterial antigens |
dc.title |
Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella
bacilliformis |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/publishedVersion |
dc.description.abstract |
BACKGROUND: Bartonella bacilliformis is the causative agent of
Carrion's disease, a neglected illness with mortality rates of
40-85% in the absence of treatment. The lack of a diagnostic
technique to overcome misdiagnosis and treat asymptomatic
carriers is of note. This study aimed to identify new B.
bacilliformis antigenic candidates that could lead to a new
diagnostic tool able to be implemented in endemic rural areas.
METHODOLOGY/PRINCIPAL FINDINGS: Blood (n = 198) and serum (n =
177) samples were collected in northern Peru. Clinical data were
recorded. Specific 16S rRNA amplification by RT-PCR, IFA and
ELISA for IgM/IgG with whole cells as antigens was done. Western
blot analysis and N-terminal amino acid sequencing detected
seroreactive proteins. ELISAs for IgM/IgG for the antigenic
candidates were performed. Of the population 33.3% reported at
least one symptom compatible with Carrion's disease; 25.4%
(IFA), 27.1% (ELISA-IgG), 33.9% (ELISA-IgM) and 38.9% (RT-PCR)
of samples were positive. Four proteins were considered
potential antigenic candidates, including two new antigenic
candidates, succinyl-CoA synthetase subunit alpha (SCS-alpha)
and succinyl-CoA synthetase subunit beta (SCS-beta). On Western
blot both Pap31 and SCS-alpha interacted with IgM, while GroEL
and SCS-beta interacted with IgG. The presence of specific
antibodies against the antigenic candidates varied from 34.5%
(IgG against SCS-alpha) to 97.2% (IgM against Pap31).
CONCLUSIONS/SIGNIFICANCE: RT-PCR and the high levels of
positivity for specific ELISAs demonstrate high levels of B.
bacilliformis exposure and asymptomatic carriers among
inhabitants. The new antigens identified might be used as a new
rapid diagnostic tool to diagnose acute Carrion's disease and
identify asymptomatic carriers. |