Title:
|
Imaging amylopectin's order in starch using 3-dimensional polarization SHG
|
Author:
|
Psilodimitrakopoulos, Sotiris; Amat Roldán, Iván; Artigas García, David; Loza Álvarez, Pablo
|
Other authors:
|
Universitat Politècnica de Catalunya. Departament de Teoria del Senyal i Comunicacions; Universitat Politècnica de Catalunya. FOTONICA - Grup de Recerca de Fotònica |
Abstract:
|
In minimally destructive SHG biomedical imaging (high resolution optical slicing) is greatly desirable to extract the
maximum of information from the light matter interaction. Here we develop a 3-D biophysical model and a
methodology, which extracts molecular information below the experimental resolution limit. Firstly, it provides the pitch
angle (SHG effective orientation) of the SHG source helix of the sample. This information is used to characterize and
categorize the SHG sources among them. And secondly, it provides the degree of organization of the SHG source
molecules. This can be used as a quantitative imaging biomarker able to characterize the degree of organization
(homeostasis) of the sample. Here we applied the model in dried and hydrated wheat starch granules. Our results show
that the SHG source molecule in starch is amylopectin. We also conclude that under hydration, the amylopectin
molecules are further organized but they do not change structure. This organization is reflected to the width of the pitch
angles pixels’ histograms’ distributions. The shorter the width is, the more organized the amylopectin molecules in starch
are. |
Abstract:
|
Peer Reviewed |
Subject(s):
|
-Àrees temàtiques de la UPC::So, imatge i multimèdia::Creació multimèdia::Imatge digital -Àrees temàtiques de la UPC::Enginyeria de la telecomunicació -Image processing -Starch -Hydration -Polarization SHG imaging -Helical pitch angle -Amylopectin -Histograms’ width -Organization -Imatges -- Processament |
Rights:
|
|
Document type:
|
Article - Published version Conference Object |
Published by:
|
International Society for Photo-Optical Instrumentation Engineers (SPIE)
|
Share:
|
|