Títol:
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Aeromonas surface glucan attached throught the O-antigen ligase represents a new way to obtain UDP-Glucose
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Autor/a:
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Merino Montero, Susana; Bouamama, Lamiaa; Knirel, Yuriy A.; Shenchenkova, Sofya N.; Regué Queralt, Miguel, 1953-; Tomàs Magaña, Juan
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Altres autors:
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Universitat de Barcelona |
Abstract:
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We previously reported that A. hydrophila GalU mutants were still able to produce UDP-glucose introduced as a glucose residue in their lipopolysaccharide core. In this study, we found the unique origin of this UDP-glucose from a branched α-glucan surface polysaccharide. This glucan, surface attached through the O-antigen ligase (WaaL), is common to the mesophilic Aeromonas strains tested. The Aeromonas glucan is produced by the action of the glycogen synthase (GlgA) and the UDP-Glc pyrophosphorylase (GlgC), the latter wrongly indicated as an ADP-Glc pyrophosphorylase in the Aeromonas genomes available. The Aeromonas glycogen synthase is able to react with UDP or ADP-glucose, which is not the case of E. coli glycogen synthase only reacting with ADP-glucose. The Aeromonas surface glucan has a role enhancing biofilm formation. Finally, for the first time to our knowledge, a clear preference on behalf of bacterial survival and pathogenesis is observed when choosing to produce one or other surface saccharide molecules to produce (lipopolysaccharide core or glucan). |
Matèries:
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-Bacteris -Glucosa -Polisacàrids -Enzims -Reacció en cadena de la polimerasa -Bacteria -Glucose -Polysaccharides -Enzymes -Polymerase chain reaction |
Drets:
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cc-by (c) Merino Montero, Susana et al., 2012
http://creativecommons.org/licenses/by/3.0/es |
Tipus de document:
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Article Article - Versió publicada |
Publicat per:
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Public Library of Science (PLoS)
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