dc.contributor.author |
Montaner-Tarbes, Sergio |
dc.contributor.author |
Borràs, Francesc E. |
dc.contributor.author |
Montoya, Maria |
dc.contributor.author |
Fraile, Lorenzo |
dc.contributor.author |
Portillo Obando, Hernando A. del |
dc.date |
2016-06-17T08:10:57Z |
dc.date |
2016-06-17T08:10:57Z |
dc.date |
2016-05-31 |
dc.date |
2016-06-13T16:01:19Z |
dc.identifier.citation |
0928-4249 |
dc.identifier.uri |
http://hdl.handle.net/2445/99583 |
dc.format |
10 p. |
dc.format |
application/pdf |
dc.language.iso |
eng |
dc.publisher |
BioMed Central |
dc.relation |
Reproducció del document publicat a:
http://dx.doi.org/10.1186/s13567-016-0345-x |
dc.relation |
Veterinary Research, 2016, vol. 47, num. 59 |
dc.relation |
http://dx.doi.org/10.1186/s13567-016-0345-x |
dc.rights |
cc by (c) Montaner-Tarbes et al., 2016 |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.rights |
http://creativecommons.org/licenses/by/3.0/es/ |
dc.subject |
Microbiologia mèdica |
dc.subject |
Malalties infeccioses |
dc.subject |
Medical microbiology |
dc.subject |
Communicable diseases |
dc.title |
Serum-derived exosomes from non-viremic animals previously
exposed to the porcine respiratory and reproductive virus
contain antigenic viral proteins |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/publishedVersion |
dc.description.abstract |
PRRSV is the etiological agent of one of the most important
swine diseases with a significant economic burden worldwide and
limitations in vaccinology. Exosomes are 30-100 nm vesicles of
endocytic origin. Remarkably, immunizations with exosomes
containing antigens from tumors or pathogens are capable of
eliciting protective immune responses, albeit variably, in
cancer and infectious diseases. Here we describe the isolation,
molecular composition and immunogenicity of serum-derived
exosomes from naive animals, from PRRSV viremic animals and from
animals previously PRRSV infected but already free of viruses
(non viremic). Exosomes were isolated through size exclusion
chromatography and characterized by different methodologies.
Exosome-enriched fractions from naive and natural infected
animals contained classical tetraspanin exosomal markers (CD63
and CD81) and high concentrations of particles in the size-range
of exosomes as detected by nanoparticle tracking analysis and
cryo-TEM. NanoLC-MS/MS was used to identify viral antigens
associated to exosomes. PRRSV-proteins were detected in serum
samples from only viremic animals and from animals previously
infected already free of viruses (non-viremic), but not in
controls. Moreover, immune sera from pigs previously exposed to
PRRSV specifically reacted against exosomes purified from
non-viremic pig sera in a dose-dependent manner, a reactivity
not detected when naive sera was used in the assay. To
facilitate future studies, a scaling-up process was implemented.
To the best of our knowledge, this is the first molecular
characterization of serum-derived exosomes from naive pigs and
pigs actively or previously infected with PRRSV. The presence of
antigenic viral proteins in serum-derived exosomes free of
virus, suggest their use as a novel vaccine approach against
PRRSV. |