dc.contributor.author |
Buonfrate, Dora |
dc.contributor.author |
Sequi, Marco |
dc.contributor.author |
Mejia, Rojelio |
dc.contributor.author |
Cimino, Ruben O. |
dc.contributor.author |
Krolewiecki, Alejandro J. |
dc.contributor.author |
Albonico, Marco |
dc.contributor.author |
Degani, Monica |
dc.contributor.author |
Tais, Stefano |
dc.contributor.author |
Angheben, Andrea |
dc.contributor.author |
Requena-Méndez, Ana |
dc.contributor.author |
Muñoz, José |
dc.contributor.author |
Nutman, Thomas B. |
dc.contributor.author |
Bisoffi, Zeno |
dc.date |
2016-01-14T12:35:25Z |
dc.date |
2016-01-14T12:35:25Z |
dc.date |
2015-02-10 |
dc.date |
2015-12-16T16:38:42Z |
dc.identifier.citation |
1935-2727 |
dc.identifier.citation |
685931 |
dc.identifier.uri |
http://hdl.handle.net/2445/68719 |
dc.format |
12 p. |
dc.format |
application/pdf |
dc.language.iso |
eng |
dc.publisher |
Public Library of Science (PLoS) |
dc.relation |
Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pntd.0003491 |
dc.relation |
PLoS Neglected Tropical Diseases, 2015, vol. 9, num. 2, p. e0003491 |
dc.relation |
http://dx.doi.org/10.1371/journal.pntd.0003491 |
dc.rights |
CC0 Buonfrate, 2015 |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.rights |
http://creativecommons.org/publicdomain/zero/1.0/ |
dc.subject |
Nematodes |
dc.subject |
Serodiagnòstic |
dc.subject |
Diagnòstic de laboratori |
dc.subject |
Helmintiasi |
dc.subject |
Nematodes |
dc.subject |
Serodiagnosis |
dc.subject |
Laboratory diagnosis |
dc.subject |
Helminthiasis |
dc.title |
Accuracy of Five Serologic Tests for the Follow up of Strongyloides stercoralis Infection |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/publishedVersion |
dc.description.abstract |
BACKGROUND: Traditional faecal-based methods have poor
sensitivity for the detection of S. stercoralis, therefore are
inadequate for post-treatment evaluation of infected patients
who should be carefully monitored to exclude the persistence of
the infection. In a previous study, we demonstrated high
accuracy of five serology tests for the screening and diagnosis
of strongyloidiasis. Aim of this study is to evaluate the
performance of the same five tests for the follow up of patients
infected with S. stercoralis. METHODS: Retrospective study on
anonymized, cryo-preserved samples available at the Centre for
Tropical Diseases (Negrar, Verona, Italy). Samples were
collected before and from 3 to 12 months after treatment. The
samples were tested with two commercially-available ELISA tests
(IVD, Bordier), two techniques based on a recombinant antigen
(NIE-ELISA and NIE-LIPS) and one in-house IFAT. The results of
each test were evaluated both in relation to the results of
fecal examination and to those of a composite reference standard
(classifying as positive a sample with positive stools and/or at
least three positive serology tests). The associations between
the independent variables age and time and the dependent
variable value of serological test (for all five tests), were
analyzed by linear mixed-effects regression model. RESULTS: A
high proportion of samples demonstrated for each test a
seroreversion or a relevant decline (optical density/relative
light units halved or decrease of at least two titers for IFAT)
at follow up, results confirmed by the linear mixed effects
model that showed a trend to seroreversion over time for all
tests. In particular, IVD-ELISA (almost 90% samples demonstrated
relevant decline) and IFAT (almost 87%) had the best
performance. Considering only samples with a complete
negativization, NIE-ELISA showed the best performance (72.5%
seroreversion). CONCLUSIONS: Serology is useful for the follow
up of patients infected with S. stercoralis and determining test
of cure. |