An efficient and reproducible method to culture Bergmann and cortical radial glia using textured PMMA

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To access the full text documents, please follow this link: http://hdl.handle.net/2117/24863

dc.contributor	Universitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica
dc.contributor	Institut de Bioenginyeria de Catalunya
dc.contributor	Universitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits
dc.contributor.author	Álvarez Pinto, Zaída
dc.contributor.author	Sena, Elena
dc.contributor.author	Mattotti, Marta
dc.contributor.author	Engel López, Elisabeth
dc.contributor.author	Alcantara, Soledad
dc.date	2014-07-30
dc.identifier.citation	Álvarez, Z. [et al.]. An efficient and reproducible method to culture Bergmann and cortical radial glia using textured PMMA. "Journal of neuroscience methods", 30 Juliol 2014, vol. 232, p. 93-101.
dc.identifier.citation	0165-0270
dc.identifier.citation	10.1016/j.jneumeth.2014.05.011
dc.identifier.uri	http://hdl.handle.net/2117/24863
dc.language.iso	eng
dc.relation	http://www.sciencedirect.com/science/article/pii/S0165027014001654#
dc.rights	Attribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subject	Àrees temàtiques de la UPC::Enginyeria dels materials
dc.subject	Biomedical materials
dc.subject	Astrocytes
dc.subject	Radial glia
dc.subject	Bergmann glia
dc.subject	Progenitors
dc.subject	Poly-methyl methacrylate (PMMA)
dc.subject	Micro-patterning
dc.subject	Surface topography
dc.subject	Materials biomèdics
dc.title	An efficient and reproducible method to culture Bergmann and cortical radial glia using textured PMMA
dc.type	info:eu-repo/semantics/publishedVersion
dc.type	info:eu-repo/semantics/article
dc.description.abstract	Radial glia cells comprise the principal population of neural stem cells (NSC) during development. Attempts to develop reproducible radial glia and NSC culture methods have met with variable results, yielding non-adherent cultures or requiring the addition of growth factors. Recent studies demonstrated that a 2-µm patterned poly-methyl methacrylate (ln2 PMMA) grooved scaffold, by mimicking the biophysical and microtopographic properties of the embryonic NSC niche, induces the de-differentiation of glial cells into functional radial glia cell
dc.description.abstract	Peer Reviewed

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