In this chapter we provide different methodologies to assess the antimicrobial activity of various substrates for industrial application. The capacity of microorganisms to colonize or remain viable on a variety of media: paintings, all kinds of plastic, wood, stone, concrete, etc., can produced a deterioration and even the possible role of these substrates as infection vectors processes in humans and animals. In this sense and based on Standards of International control we have developed a simple and reliable method to verify, if the ability of individual carriers treated with natural products with inhibitory capacity of microorganisms to prevent, delay and/or minimize its biodeterioration.

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