Title:
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Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy
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Author:
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Aviles Espinosa, Rodrigo; Andilla, Jordi; Porcar-Guezenec, Rafael; Olarte, Omar E.; Nieto, Marta; Levecq, Xavier; Artigas García, David; Loza Álvarez, Pablo
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Other authors:
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Universitat Politècnica de Catalunya. Departament de Teoria del Senyal i Comunicacions; Universitat Politècnica de Catalunya. FOTONICA - Grup de Recerca de Fotònica |
Abstract:
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We demonstrate that sample induced aberrations can be measured in a nonlinear microscope. This uses the fact that two-photon excited fluorescence naturally produces a localized point source inside the sample: the nonlinear guide-star (NL-GS). The wavefront emitted from the NL-GS can then be recorded using a Shack-Hartmann sensor. Compensation of the recorded sample aberrations is performed by the deformable mirror in a single-step. This technique is applied to fixed and in vivo biological samples, showing, in some cases, more than one order of magnitude improvement in the total collected signal intensity. |
Abstract:
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Peer Reviewed |
Subject(s):
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-Àrees temàtiques de la UPC::Física -Nonlinear optics -Adaptative optics -Multiphoton microscopy -Spherical-aberration -Deep-Tissue -Òptica no lineal |
Rights:
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Document type:
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Article - Published version Article |
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