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Título:
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Differential interactions of virulent and non-virulent H. parasuis strains with naive or swine influenza virus pre-infected dendritic cells
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Autor/a:
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Mussá, Tufária; Rodríguez-Cariño, Carolina; Sánchez-Chardi, Alejandro; Baratelli, Massimiliano; Costa-Hurtado, Mar; Fraile Sauce, Lorenzo José; Domínguez, Javier; Aragon, Virginia; Montoya, Maria
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Notas:
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Pigs possess a microbiota in the upper respiratory tract that includes Haemophilus parasuis. Pigs are also considered
the reservoir of influenza viruses and infection with this virus commonly results in increased impact of bacterial
infections, including those by H. parasuis. However, the mechanisms involved in host innate responses towards
H. parasuis and their implications in a co-infection with influenza virus are unknown. Therefore, the ability of a
non-virulent H. parasuis serovar 3 (SW114) and a virulent serovar 5 (Nagasaki) strains to interact with porcine bone
marrow dendritic cells (poBMDC) and their modulation in a co-infection with swine influenza virus (SwIV) H3N2 was
examined. At 1 hour post infection (hpi), SW114 interaction with poBMDC was higher than that of Nagasaki, while
at 8 hpi both strains showed similar levels of interaction. The co-infection with H3N2 SwIV and either SW114 or
Nagasaki induced higher levels of IL-1β, TNF-α, IL-6, IL-12 and IL-10 compared to mock or H3N2 SwIV infection
alone. Moreover, IL-12 and IFN-α secretion differentially increased in cells co-infected with H3N2 SwIV and Nagasaki.
These results pave the way for understanding the differences in the interaction of non-virulent and virulent strains
of H. parasuis with the swine immune system and their modulation in a viral co-infection.
This work was partly funded by the following Projects: CSD 2006–00007,
AGL2009-12945-C02-01, AGL2010-22200-C02-01 and AGL2010-15232 by the Spanish Government. PhD studies of Mrs. Tufária Mussá are supported by a
doctoral grant from the Spanish Agency of International Cooperation and
Development (AECID) while PhD studies of Mrs. Mar Costa-Hurtado and Mr.
Massimiliano Baratelli are supported by a doctoral grant from the Spanish
Ministry of Science and Innovation (MICINN) |
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Derechos:
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cc-by (c) Mussá, Tufária et al., 2012
http://creativecommons.org/licenses/by/3.0/es/
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Tipo de documento:
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article
publishedVersion |
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Editor:
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BioMed Central
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