| dc.contributor.author |
Sangalli-Leite, Fernanda |
| dc.contributor.author |
Scorzoni, Liliana |
| dc.contributor.author |
Mesa-Arango, Ana Cecilia |
| dc.contributor.author |
Casas Herranz, Celia |
| dc.contributor.author |
Herrero Perpiñán, Enrique |
| dc.contributor.author |
Mendes Gianinni, Maria José Soares |
| dc.contributor.author |
Rodríguez-Tudela, Juan Luis |
| dc.contributor.author |
Cuenca-Estrella, Manuel |
| dc.contributor.author |
Zaragoza, Oscar |
| dc.date |
2015-07-07T11:22:29Z |
| dc.date |
2011 |
| dc.date |
10000-01-01 |
| dc.identifier |
1286-4579 |
| dc.identifier |
http://hdl.handle.net/10459.1/48424 |
| dc.identifier |
https://doi.org/10.1016/j.micinf.2011.01.015 |
| dc.identifier.uri |
http://hdl.handle.net/10459.1/48424 |
| dc.description |
We studied the effects of Amphotericin B (AmB) on Cryptococcus neoformans using different viability methods (CFUs enumeration, XTT
assay and propidium iodide permeability). After 1 h of incubation, there were no viable colonies when the cells were exposed to AmB concentrations ≥ 1 mg/L. In the same conditions, the cells did not become permeable to propidium iodide, a phenomenon that was not observed
until 3 h of incubation. When viability was measured in parallel using XTT assay, a result consistent with the CFUs was obtained, although we
also observed a paradoxical effect in which at high AmB concentrations, a higher XTT reduction was measured than at intermediate AmB
concentrations. This paradoxical effect was not observed after 3 h of incubation with AmB, and lack of XTT reduction was observed at AmB
concentrations higher than 1 mg/L. When stained with dihydrofluorescein, AmB induced a strong intracellular oxidative burst. Consistent with
oxidative damage, AmB induced protein carbonylation. Our results indicate that in C. neoformans, Amphotericin B causes intracellular damage mediated through the production of free radicals before damage on the cell membrane, measured by propidium iodide uptake. |
| dc.language |
eng |
| dc.publisher |
Elsevier |
| dc.relation |
Reproducció del document publicat a https://doi.org/10.1016/j.micinf.2011.01.015 |
| dc.relation |
Microbes and Infection, 2011, vol. 13, núm. 5 , p. 457-467 |
| dc.rights |
(c) Elsevier, 2011 |
| dc.rights |
(c) Institut Pasteur, 2011 |
| dc.rights |
info:eu-repo/semantics/restrictedAccess |
| dc.subject |
Cryptococcus neoformans |
| dc.subject |
Amphotericin B |
| dc.subject |
Oxidative burst |
| dc.title |
Amphotericin B mediates killing in Cryptococcus neoformans through the induction of a strong oxidative burst |
| dc.type |
article |
| dc.type |
publishedVersion |
