|
Título:
|
Characterizing the proteome and oxi-proteome of apple in response to a compatible (Penicillium expansum) and a non-host (P. digitatum) pathogen.
|
|
Autor/a:
|
Burón Moles, Gemma; Wisniewski, Michae; Viñas Almenar, Inmaculada; Teixidó i Espasa, Neus; Usall i Rodié, Josep; Droby, Samir; Torres Sanchis, Rosario
|
|
Notas:
|
Apples are subjected to both abiotic and biotic stresses during the postharvest period, which lead to large economic losses worldwide. To obtain biochemical insights into apple defense response, we monitored the protein abundance changes (proteome), as well as the protein carbonyls (oxi-proteome) formed by reactive oxygen species (ROS) in"Golden Smoothee" apple in response to wounding, Penicillium expansum (host) and Penicillium digitatum (non-host) pathogens with select transcriptional studies. To examine the biological relevance of the results, we described quantitative and oxidative protein changes into the gene ontology functional categories, as well as into de KEGG pathways. We identified 26 proteins that differentially changed in abundance in response to wounding, P. expansum or P. digitatum infection. While these changes showed some similarities between the apple responses and abiotic and biotic stresses, Mal d 1.03A case, other proteins as Mal d 1.03E and EF-Tu were specifically induced in response to P. digitatum infection. Using a protein carbonyl detection method based on fluorescent Bodipy, we detected and identified 27 oxidized proteins as sensitive ROS targets. These ROS target proteins were related to metabolism processes, suggesting that this process plays a leading role in apple fruit defense response against abiotic and biotic stresses. ACC oxidase and two glutamine synthetases showed the highest protein oxidation level in response to P. digitatum infection. Documenting changes in the proteome and, specifically in oxi-proteome of apple can provide information that can be used to better understand how impaired protein functions may affect apple defense mechanisms. Possible mechanisms by which these modified proteins are involved in fruit defense response are discussed. |
|
Materia(s):
|
-Malus x domestica
-Green mold
-Blue mold
-protein oxidation
-RT-qPCR
-Pomes
-Tecnologia postcollita
-Proteïnes dels aliments
-Apples
-Postharvest technology
-Protein content of food |
|
Derechos:
|
(c) Elsevier, 2015
info:eu-repo/semantics/restrictedAccess |
|
Tipo de documento:
|
Artículo
Artículo - Versión publicada |
|
Editor:
|
Elsevier
|
|
Compartir:
|
|
