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Título:
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Can eukaryotic cells monitor the presence of unreplicated DNA?
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Autor/a:
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Torres Rosell, Jordi; Piccoli, Giacomo De; Aragón, Luis
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Notas:
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Completion of DNA replication before mitosis is essential for genome stability and cell viability.
Cellular controls called checkpoints act as surveillance mechanisms capable of detecting errors and
blocking cell cycle progression to allow time for those errors to be corrected. An important
question in the cell cycle field is whether eukaryotic cells possess mechanisms that monitor ongoing
DNA replication and make sure that all chromosomes are fully replicated before entering mitosis,
that is whether a replication-completion checkpoint exists. From recent studies with smc5–smc6
mutants it appears that yeast cells can enter anaphase without noticing that replication in the
ribosomal DNA array was unfinished. smc5–smc6 mutants are proficient in all known cellular
checkpoints, namely the S phase checkpoint, DNA-damage checkpoint, and spindle checkpoint,
thus suggesting that none of these checkpoints can monitor the presence of unreplicated segments
or the unhindered progression of forks in rDNA. Therefore, these results strongly suggest that
normal yeast cells do not contain a DNA replication-completion checkpoint. |
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Derechos:
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cc-by, (c) Torres-Rosell et al., 2007
http://creativecommons.org/licenses/by/2.0/
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Tipo de documento:
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article
publishedVersion |
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Editor:
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BioMed Central
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