dc.contributor.author |
David, Mónica Delia |
dc.contributor.author |
Cantí Nicolás, Carles |
dc.contributor.author |
Herreros Danés, Judit |
dc.date |
2014-06-20T12:42:38Z |
dc.date |
2014-06-20T12:42:38Z |
dc.date |
2010 |
dc.identifier |
0360-4012 |
dc.identifier |
http://hdl.handle.net/10459.1/47338 |
dc.identifier |
https://doi.org/10.1002/jnr.22464 |
dc.identifier.uri |
http://hdl.handle.net/10459.1/47338 |
dc.description |
Wnt factors regulate neural stem cell development and neuronal connectivity. Here we investigated whether Wnt-3a and Wnt-3, expressed in the developing spinal cord, regulate proliferation and the neuronal differentiation of spinal cord neural precursors (SCNP). Wnt-3a promoted a sustained increase of SCNP proliferation, whereas Wnt-3 enhanced SCNP proliferation transiently and increased neurogenesis through β-catenin signaling. Consistent with this, Wnt-3a and Wnt-3 differently regulate the expression of Cyclin-dependent kinase inhibitors. Furthermore, Wnt-3a and Wnt-3 stimulated neurite outgrowth in SCNP-derived neurons through ß-catenin and TCF4-dependent transcription. GSK-3ß inhibitors mimicked Wnt signaling and promoted neurite outgrowth in established cultures. We conclude that Wnt-3a and Wnt-3 signal through the canonical Wnt/β-catenin pathway to regulate different aspects of SCNP development. These findings may be of therapeutic interest for the treatment of neurodegenerative diseases and nerve injury. |
dc.language |
eng |
dc.publisher |
Wiley |
dc.relation |
Versió preprint del document publicat a: https://doi.org/10.1002/jnr.22464 |
dc.relation |
Journal of Neuroscience Research, 2010, vol. 88, núm. 14, p. 3011-3023 |
dc.rights |
(c) Wiley, 2010 |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
Neural stem cells |
dc.subject |
Neuronal differentiation |
dc.subject |
Glycogen-synthase-kinase |
dc.subject |
Sistema nerviós |
dc.title |
Wnt-3a and Wnt-3 differently stimulate proliferation and neurogenesis of spinal neural precursors and promote neurite outgrowth by canonical signaling |
dc.type |
article |
dc.type |
submittedVersion |