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Title:
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Influence of sample processing on the analysis of carotenoids in maize
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Author:
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Rivera Vélez, Sol Maiam; Canela i Garayoa, Ramon
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Notes:
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We performed a number of tests with the aim to develop an effective extraction
method for the analysis of carotenoid content in maize seed. Mixtures of methanol–ethyl acetate (6:4, v/v) and methanol–tetrahydrofuran (1:1, v/v) were the most effective solvent systems for carotenoid extraction from maize endosperm under the conditions assayed. In addition, we also addressed sample preparation prior to the analysis of carotenoids by liquid chromatography (LC). The LC response of extracted carotenoids and standards in several solvents was evaluated and results were related to the degree of solubility of these pigments. Three key factors were found to be important when selecting a suitable injection solvent: compatibility between the mobile phase and injection solvent, carotenoid polarity
and content in the matrix.
This work was supported by the University of Lleida, Ministry of Science and Innovation (MICINN), Spain (BFU200761413 and CTQ2009-14699-C02-01), and an ERC Advanced Grant (BIOFORCE) to Paul Christou. |
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Subject(s):
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-Carotenoids
-Extraction
-Solubility
-Injection solvent
-Liquid chromatography
-Blat de moro -- Anàlisi |
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Rights:
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cc-by, (c) Rivera et al., 2012
http://creativecommons.org/licenses/by/3.0/es/deed.ca
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Document type:
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article
publishedVersion |
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Published by:
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Molecular Diversity Preservation International
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Share:
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