2026-04-14T08:46:01Zhttps://recercat.cat/oai/requestoai:recercat.cat:2445/980512025-12-04T19:38:22Zcom_2072_1057col_2072_478813col_2072_478917
RECERCAT
author
Para, B. V. (Bianca Veronica)
author
Núñez Burcio, Oscar
author
Moyano Morcillo, Encarnación
author
Galcerán Huguet, M. Teresa
2016-04-29T11:18:30Z2016-04-29T11:18:30Z2006-05-302016-04-29T11:18:36Z
Conditions for the separation and determination of benzalkonium chloride (BAC) homologues by CE with UV-detection and CE coupled to MS (IT) using electrospray as ionization source were established. The separation was performed using fused-silica capillaries of 50 μm id and 100 mM acetic acid-ammonium acetate buffer solution at pH 4.5 with 80% of ACN as carrier electrolyte. CE-MS coupling parameters were optimized and methanol-10 mM acetic acid (90:10 v/v) was selected as sheath liquid. Detection limits, based on an S/N of 3:1, were calculated, and values between 0.8 and 1.3 mg/L with CE-ESI/MS and around 0.5 mg/L with CE-ESI-MS/MS, using hydrodynamic injection (15 s, 3.5 kPa), were obtained. Good run-to-run and day-to-day precisions on concentration were achieved with RSDs lower than 8%. Quantitative analysis was carried out by the internal standard method and the calibration curves showed good linearities (r2>0.98). The CE-ESI-MS/MS method was successfully applied to the analysis of BAC in different ophthalmic solutions, allowing the direct determination, identification and confirmation of the BAC homologues presented in these samples.
(c) Wiley-VCH, 2006 info:eu-repo/semantics/openAccess
Electroforesi capil·lar
Capillary electrophoresis
Análisis of Benzalkonium chloride by capillary electrophoresis-electrospray-Tandem mass spectrometry
info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion