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oai:recercat.cat:2445/980512025-12-04T19:38:22Zcom_2072_1057col_2072_478813col_2072_478917

urn:hdl:2445/98051 Análisis of Benzalkonium chloride by capillary electrophoresis-electrospray-Tandem mass spectrometry Para, B. V. (Bianca Veronica) Núñez Burcio, Oscar Moyano Morcillo, Encarnación Galcerán Huguet, M. Teresa Electroforesi capil·lar Capillary electrophoresis Conditions for the separation and determination of benzalkonium chloride (BAC) homologues by CE with UV-detection and CE coupled to MS (IT) using electrospray as ionization source were established. The separation was performed using fused-silica capillaries of 50 μm id and 100 mM acetic acid-ammonium acetate buffer solution at pH 4.5 with 80% of ACN as carrier electrolyte. CE-MS coupling parameters were optimized and methanol-10 mM acetic acid (90:10 v/v) was selected as sheath liquid. Detection limits, based on an S/N of 3:1, were calculated, and values between 0.8 and 1.3 mg/L with CE-ESI/MS and around 0.5 mg/L with CE-ESI-MS/MS, using hydrodynamic injection (15 s, 3.5 kPa), were obtained. Good run-to-run and day-to-day precisions on concentration were achieved with RSDs lower than 8%. Quantitative analysis was carried out by the internal standard method and the calibration curves showed good linearities (r2>0.98). The CE-ESI-MS/MS method was successfully applied to the analysis of BAC in different ophthalmic solutions, allowing the direct determination, identification and confirmation of the BAC homologues presented in these samples. 2016-04-29T11:18:30Z 2016-04-29T11:18:30Z 2006-05-30 2016-04-29T11:18:36Z info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Versió postprint del document publicat a: http://dx.doi.org/10.1002/elps.200500716 Electrophoresis, 2006, vol. 27, num. 11, p. 2225-2232 http://dx.doi.org/10.1002/elps.200500716 (c) Wiley-VCH, 2006 info:eu-repo/semantics/openAccess Wiley-VCH Articles publicats en revistes (Enginyeria Química i Química Analítica)