2026-04-17T06:47:50Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/693692025-12-05T13:17:40Zcom_2072_1057col_2072_478798col_2072_478916col_2072_478917

urn:hdl:2445/69369 Detection of Streptococcus pneumoniae and Haemophilus influenzae type B by real-time PCR from dried blood spot samples among children with pneumonia: a useful approach for developing countries Selva Jové, Laura Benmessaoud, Rachid Lanaspa, Miguel Jroundi, Imane Moraleda Redecilla, Cinta Acácio, Sozinho Iñigo, Melania Bastiani, Alien Monsonís, Manuel Pallarés Giner, Roman Bassat Orellana, Quique Muñoz-Almagro, Carmen Bacteris patògens Reacció en cadena de la polimerasa Pneumònia Infants Pneumococs Pathogenic bacteria Polymerase chain reaction Pneumonia Children Streptococcus pneumonia Correction https://doi.org/10.1371/journal.pone.0147678 Background: Dried blood spot (DBS) is a reliable blood collection method for storing samples at room temperature and easily transporting them. We have previously validated a Real-Time PCR for detection of Streptococcus pneumoniae in DBS. The objective of this study was to apply this methodology for the diagnosis of S. pneumoniae and Haemophilus influenzae b (Hib) in DBS samples of children with pneumonia admitted to two hospitals in Mozambique and Morocco. Methods: Ply and wzg genes of S. pneumoniae and bexA gene of Hib, were used as targets of Real-Time PCR. 329 DBS samples of children hospitalized with clinical diagnosis of pneumonia were tested. Results: Real-Time PCR in DBS allowed for a significant increase in microbiological diagnosis of S. pneumoniae and Hib. When performing blood bacterial culture, only ten isolates of S. pneumoniae and none of Hib were detected (3·0% positivity rate, IC95% 1·4-5·5%). Real-Time PCR from DBS samples increased the detection yield by 4x fold, as 30 S. pneumoniae and 11 Hib cases were detected (12·4% positivity rate, IC95% 9·0-16·5%; P<0·001). Conclusion: Real-Time PCR applied in DBS may be a valuable tool for improving diagnosis and surveillance of pneumonia caused by S. pneumoniae or Hib in developing countries. 2016-02-10T15:17:51Z 2016-02-10T15:17:51Z 2013-10-08 2016-02-10T15:17:51Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0076970 PLoS One, 2013, vol. 8, num. 10, p. e76970 http://dx.doi.org/10.1371/journal.pone.0076970 cc-by (c) Selva Jové, Laura et al., 2013 http://creativecommons.org/licenses/by/3.0/es info:eu-repo/semantics/openAccess Public Library of Science (PLoS) Articles publicats en revistes (Ciències Clíniques)