2026-04-13T04:08:42Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/662152025-12-05T16:27:40Zcom_2072_1057col_2072_478917col_2072_478921col_2072_478924

00925njm 22002777a 4500 dc Vázquez Fresno, Rosa author Llorach, Rafael author Urpí Sardà, Mireia author Lupianez-Barbero, Ascensión author Estruch Riba, Ramon author Corella Piquer, Dolores author Fitó Colomer, Montserrat author Arós, Fernando author Ruiz-Canela, Miguel author Salas Salvadó, Jordi author Andrés Lacueva, Ma. Cristina author 2015-07-07T14:54:34Z 2015-10-29T23:01:42Z 2015-10-29 2015-07-07T14:54:34Z The Mediterranean diet (MD) is considered a dietary pattern with beneficial effects on human health. The aim of this study was to assess the effect of an MD on urinary metabolome by comparing subjects at 1 and 3 years of follow-up, after an MD supplemented with either extra-virgin olive oil (MD + EVOO) or nuts (MD + Nuts), to those on advice to follow a control low-fat diet (LFD). Ninety-eight nondiabetic volunteers were evaluated, using metabolomic approaches, corresponding to MD + EVOO (n = 41), MD + Nuts (n = 27), or LFD (n = 30) groups. The 1H NMR urinary profiles were examined at baseline and after 1 and 3 years of follow-up. Multivariate data analysis (OSC-PLS-DA and HCA) methods were used to identify the potential biomarker discriminating groups, exhibiting a urinary metabolome separation between MD groups against baseline and LFD. Results revealed that the most prominent hallmarks concerning MD groups were related to the metabolism of carbohydrates (3-hydroxybutyrate, citrate, and cis-aconitate), creatine, creatinine, amino acids (proline, N-acetylglutamine, glycine, branched-chain amino acids, and derived metabolites), lipids (oleic and suberic acids), and microbial cometabolites (phenylacetylglutamine and p-cresol). Otherwise, hippurate, trimethylamine-N-oxide, histidine and derivates (methylhistidines, carnosine, and anserine), and xanthosine were predominant after LFD. The application of NMR-based metabolomics enabled the classification of individuals regarding their dietary pattern and highlights the potential of this approach for evaluating changes in the urinary metabolome at different time points of follow-up in response to specific dietary interventions. Dieta Cuina mediterrània Nutrició Metabòlits Dieta sense greix Cuina (Nous) Oli d'oliva Ressonància magnètica nuclear Diet Mediterranean cooking Nutrition Metabolites Low-fat diet Cooking (Nuts) Olive oil Nuclear magnetic resonance Metabolomic pattern analysis after Mediterranean diet intervention in non-diabetic population: a 1- and 3-year follow-up in the PREDIMED study