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urn:hdl:2445/61481 A glucuronoxylan-specific xylanase from a new Paenibacillus favisporus strain isolated from tropical soil of Brazil Padilha, Itácio Q. M. Valenzuela Mayorga, Susana Valeria Grisi, Teresa Díaz Lucea, Pilar de Araújo, Demetrius Pastor Blasco, Francisco I. Javier Bacils Ecologia microbiana Enzims microbians Biopolímers Sòls Bacillus (Bacteria) Microbial ecology Microbial enzymes Biopolymers Soils A new xylanolytic strain, Paenibacillus favisporus CC02-N2, was isolated from sugarcane plantation fi elds in Brazil. The strain had a xylan-degrading system with multiple enzymes, one of which, xylanase Xyn30A, was identifi ed and characterized. The enzyme is a single-domain xylanase belonging to family 30 of the glycosyl hydrolases (GH30). Xyn30A shows high activity on glucuronoxylans, with a Vmax of 267.2 U mg<br>1, a Km of 4.0 mg/ml, and a kcat of 13,333 min<br>1 on beechwood xylan, but it does not hydrolyze arabinoxylans. The three- dimensional structure of Xyn30A consists of a common (β/α)8 barrel linked to a side-chain-associated β-structure, similar to previously characterized GH30 xylanases. The hydrolysis products from glucuronoxylan were methylglucuronic-acid-substituted xylooligomers (acidic xylooligosaccharides). The enzyme bound to insoluble xylan but not to crystalline cellulose. Our results suggest a specifi c role for Xyn30A in xylan biodegradation in natural habitats. The enzyme is a good candidate for the production of tailored xylooligosaccharides for use in the food industry and in the biotechnological transformation of biomass. [Int Microbiol 2014; 17(3):175-184] 2015-01-19T13:03:35Z 2015-01-19T13:03:35Z 2014 2015-01-19T13:03:35Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Reproducció del document publicat a: http://dx.doi.org/:10.2436/20.1501.01.220. International Microbiology, 2014, vol. 17, num. 3, p. 175-184 http://dx.doi.org/10.2436/20.1501.01.220. cc-by-nc-sa (c) Spanish Society for Microbiology (SEM) and Viguera Editores SL, 2014 http://creativecommons.org/licenses/by-nc-sa/3.0/es info:eu-repo/semantics/openAccess Spanish Society for Microbiology (SEM) and Viguera Editores SL Articles publicats en revistes (Genètica, Microbiologia i Estadística)