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oai:recercat.cat:2445/567442025-12-05T05:42:31Zcom_2072_1057col_2072_478824col_2072_478917

Efficacy of the fluorescent dyes Fast Blue, Fluoro-Gold, and Diamidino Yellow for retrograde tracing to dorsal root ganglia after subcutaneous injection Puigdellívol Sánchez, Anna Prats Galino, Alberto Ruano Gil, Domingo Molander, Carl Transport biològic Nervis espinals Pell Rates (Animals de laboratori) Biological transport Spinal nerves Skin Rats as laboratory animals The present study was designed to investigate the efficacy of the fluorescent dyes Fast Blue (FB), Fluoro-Gold (FG), and Diamidino Yellow (DY) for retrograde tracing of lumbar dorsal root ganglia after their subcutaneous injection into different hindlimb digits. Injection of equal volumes (0.5 mu l) of 5% FB or 2% FG resulted in similar mean numbers of sensory neurones labelled by each tracer. Injection of equal volumes (0.5 mu l) of FB or FG in a single digit followed 10 days later by a second injection of the same volume of 5% DY into the same digit resulted in similar mean numbers of labelled sensory neurones for each of the three tracers. Furthermore, on average, 75% of all the FB-labelled cells and 74% of all FC-labelled cells also contained DY. Repeating the same experiment with an increased volume of DY (1.5 mu l) resulted in an increase in the mean number of double-labelled profiles to 82 and 84% for FB and FG, respectively. The results show that FB, FG and DY label similar numbers of cutaneous afferents and that a high level of double labelling may be obtained after sequential injections in digits. These properties make them suitable candidates in investigations where a combination of tracers with similar labelling efficacies is needed. 2014-09-05T09:51:42Z 2014-09-05T09:51:42Z 1998-12-15 2014-09-05T09:51:42Z info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion 0165-0270 https://hdl.handle.net/2445/56744 502145 9894781 eng Versió postprint del document publicat a: http://dx.doi.org/10.1016/S0165-0270(98)00137-X Journal of Neuroscience Methods, 1998, vol. 86, num. 1, p. 7-16 http://dx.doi.org/10.1016/S0165-0270(98)00137-X (c) Elsevier B.V., 1998 info:eu-repo/semantics/openAccess 23 p. application/pdf Elsevier B.V. Articles publicats en revistes (Fonaments Clínics)