2026-04-19T19:21:36Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/531142025-12-05T14:55:21Zcom_2072_1057col_2072_478917col_2072_478924

00925njm 22002777a 4500 dc Romero Romero, María del Mar author Holmgren-Holm, Fredrik author Grasa Martínez, Maria del Mar author Esteve Ràfols, Montserrat author Remesar Betlloch, Xavier author Fernández López, José Antonio author Alemany, Marià, 1946- author 2014-03-28T14:50:35Z 2014-03-28T14:50:35Z 2013-02-22 2014-03-28T14:50:35Z In the metabolic syndrome, glucocorticoid activity is increased, but circulating levels show little change. Most of blood glucocorticoids are bound to corticosteroid-binding globulin (CBG), which liver expression and circulating levels are higher in females than in males. Since blood hormones are also bound to blood cells, and the size of this compartment is considerable for androgens and estrogens, we analyzed whether sex or eating a cafeteria diet altered the compartmentation of corticosterone in rat blood. The main corticosterone compartment in rat blood is that specifically bound to plasma proteins, with smaller compartments bound to blood cells or free. Cafeteria diet increased the expression of liver CBG gene, binding plasma capacity and the proportion of blood cell-bound corticosterone. There were marked sex differences in blood corticosterone compartmentation in rats, which were unrelated to testosterone. The use of a monoclonal antibody ELISA and a polyclonal Western blot for plasma CBG compared with both specific plasma binding of corticosterone and CBG gene expression suggested the existence of different forms of CBG, with varying affinities for corticosterone in males and females, since ELISA data showed higher plasma CBG for males, but binding and Western blot analyses (plus liver gene expression) and higher physiological effectiveness for females. Good cross- reactivity to the antigen for polyclonal CBG antibody suggests that in all cases we were measuring CBG.The different immunoreactivity and binding affinity may help explain the marked sex-related differences in plasma hormone binding as sex-linked different proportions of CBG forms. Dieta Hormones esteroides Corticosteroides Diferències entre sexes Rates (Animals de laboratori) Diet Steroid hormones Adrenocortical hormones Sex differences Rats as laboratory animals Modulation in Wistar rats of blood corticosterone compartmentation by sex and prior exposure to a cafeteria diet