2026-04-20T03:08:50Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/2288942026-04-15T11:24:29Zcom_2072_1057col_2072_478858col_2072_478916col_2072_478917

00925njm 22002777a 4500 dc Casós, Kelly author Llatjós, Roger author Blasco Lucas, Arnau author Kuguel, Sebastián G. author Sbraga, Fabrizio author Galli, Cesare author Padler-Karavani, Vered author Le Tourneau, Thierry author Vadori, Marta author Roussel, Jean-Christian author Bottio, Tomaso author Cozzi, Emanuele author Soulillou, Jean Paul author Galiñanes, Manuel author Mañez, Rafael author Costa, Cristina author 2026-04-14T10:21:14Z 2026-04-14T10:21:14Z 2025-12-31 2026-02-24T12:34:34Z Structural valve deterioration (SVD) remains a key limitation in bioprosthetic heart valve (BHV) usage influenced by patient age. A deeper understanding of SVD pathogenesis, particularly of the immune-mediated processes altering current BHV materials, is therefore critical. To this end, commercially available BHV tissues of bovine, porcine, and equine origin were investigated following subcutaneous implantation into alpha 1,3-galactosyltransferase-knockout (Gal KO) mice. We compared the immune responses between adult and aged animals via histological assessments of explants and measurement of serum anti-galactose alpha 1,3-galactose (Gal) and anti-non-Gal antibodies at 2 months post-implantation. In contrast to adult mice, old Gal KO mice did not show increased levels of serum anti-Gal or -non-Gal antibodies after receiving specific BHV tissue (i.e., Freedom-Solo). Instead, a significant decrease in serum anti-Gal IgM was found in old recipients of Freedom-Solo. Furthermore, the overall cellular immune response was attenuated in explants from old mice compared with adults (i.e., ATS 3f and Crown). Nevertheless, the Freedom-Solo (bovine) and the Hancock-II (porcine) tissues still elicited strong cellular immune infiltration in the old cohorts. Therefore, the Gal KO mouse model offers a valuable platform to investigate age-related differences regarding cellular and humoral immune responses to various BHV tissues, contributing to our understanding of SVD. https://hdl.handle.net/2445/228894 Immunologia de la trasplantació Malalties del cor Vàlvules cardíaques Transplantation immunology Heart diseases Heart valves Antibody and Cellular Immune Responses in Old α1,3-Galactosyltransferase-Knockout Mice Implanted with Bioprosthetic Heart Valve Tissues