2026-04-20T03:08:05Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/2288942026-04-15T11:24:29Zcom_2072_1057col_2072_478858col_2072_478916col_2072_478917

2026-04-15T11:24:29Z urn:hdl:2445/228894 Antibody and Cellular Immune Responses in Old α1,3-Galactosyltransferase-Knockout Mice Implanted with Bioprosthetic Heart Valve Tissues Casós, Kelly Llatjós, Roger Blasco Lucas, Arnau Kuguel, Sebastián G. Sbraga, Fabrizio Galli, Cesare Padler-Karavani, Vered Le Tourneau, Thierry Vadori, Marta Roussel, Jean-Christian Bottio, Tomaso Cozzi, Emanuele Soulillou, Jean Paul Galiñanes, Manuel Mañez, Rafael Costa, Cristina Immunologia de la trasplantació Malalties del cor Vàlvules cardíaques Transplantation immunology Heart diseases Heart valves Structural valve deterioration (SVD) remains a key limitation in bioprosthetic heart valve (BHV) usage influenced by patient age. A deeper understanding of SVD pathogenesis, particularly of the immune-mediated processes altering current BHV materials, is therefore critical. To this end, commercially available BHV tissues of bovine, porcine, and equine origin were investigated following subcutaneous implantation into alpha 1,3-galactosyltransferase-knockout (Gal KO) mice. We compared the immune responses between adult and aged animals via histological assessments of explants and measurement of serum anti-galactose alpha 1,3-galactose (Gal) and anti-non-Gal antibodies at 2 months post-implantation. In contrast to adult mice, old Gal KO mice did not show increased levels of serum anti-Gal or -non-Gal antibodies after receiving specific BHV tissue (i.e., Freedom-Solo). Instead, a significant decrease in serum anti-Gal IgM was found in old recipients of Freedom-Solo. Furthermore, the overall cellular immune response was attenuated in explants from old mice compared with adults (i.e., ATS 3f and Crown). Nevertheless, the Freedom-Solo (bovine) and the Hancock-II (porcine) tissues still elicited strong cellular immune infiltration in the old cohorts. Therefore, the Gal KO mouse model offers a valuable platform to investigate age-related differences regarding cellular and humoral immune responses to various BHV tissues, contributing to our understanding of SVD. 2026-04-15T11:24:29Z 2026-04-15T11:24:29Z 2026-04-14T10:21:14Z 2026-04-14T10:21:14Z 2025-12-31 2026-02-24T12:34:34Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://hdl.handle.net/2445/228894 Reproducció del document publicat a: https://doi.org/10.3390/bioengineering13010053 Bioengineering, 2025, vol. 13, issue. 1, p. 53 https://doi.org/10.3390/bioengineering13010053 cc-by (c) Casós, Kelly et al. 2025 https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess MDPI AG