2026-04-17T20:31:12Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/2282192026-04-07T19:52:37Zcom_2072_1057col_2072_478917col_2072_478927

Immunological and tissue reactions to titanium particles generated by the mechanical decontamination of dental implants: In vitro and in vivo study Gil, Javier Fonseca, Dacio Fernández-Domínguez, Manuel Fernández-Domínguez, Pedro Akagi-Camacho, Sayuri Toledano Serrabona, Jorge Vegas Bustamante, Erika Camps Font, Octavi Sánchez Garcés, Ma. Ángeles Aragoneses, Juan Manuel Implants dentals Immunologia Dental implants Immunology Background: Mechanical decontamination of biofilm, or implantoplasty, is a commonly employed technique for managing peri-implantitis. However, the inflammatory response and in vivo behavior of titanium (Ti) particles released during this procedure remain underexplored. This study aimed to evaluate the cytotoxic, inflammatory, and osteogenic effects of Ti particles released during implantoplasty, as well as their in vivo behavior Material and Methods: Titanium particles were generated by following a standardized protocol using drills on 150 commercially pure Ti implants. Cytotoxicity thresholds were determined using THP-1 macrophages and bone marrow-derived mesenchymal stem cells (BM-MSCs). These cells were subsequently cultured with Ti particle-conditioned medium, and inflammatory responses were analyzed using RT-qPCR for markers such as CCR7, TNF-α, IL-1β (pro-inflammatory), and CD206, TGF-β, IL-10 (anti-inflammatory). Cytokine levels were quantified using ELISA. Osteogenic responses in BM-MSCs were assessed by analyzing Runx2, alkaline phosphatase (ALP), and osteocalcin (OC) expression, and ALP activity was measured colorimetrically. In vivo, Ti particles were introduced into mandibular defects in 20 Wistar rats, with histological analysis performed 20 days post-implantation Results: Ti particles elicited a pro-inflammatory response in macrophages, with increased expression of TNF-α and reduced expression of TGF-β and CD206. Cytokine analysis confirmed elevated IL-1β and reduced IL-10 levels. No significant changes in ALP activity were observed. Conclusions: Titanium particles released during implantoplasty induce pro-inflammatory responses. 2026-03-17T17:26:46Z 2026-03-17T17:26:46Z 2026 2026-03-17T17:26:50Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 1698-4447 https://hdl.handle.net/2445/228219 768407 41108771 eng Reproducció del document publicat a: https://doi.org/10.4317/medoral.27171. Medicina Oral Patologia Oral y Cirugia Bucal, 2026, vol. 1, p. 11-18 https://doi.org/10.4317/medoral.27171. (c) Medicina Oral SL, 2026 info:eu-repo/semantics/openAccess 8 p. application/pdf Medicina Oral SL Articles publicats en revistes (Odontoestomatologia)