2026-04-18T06:00:06Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/2280602026-04-08T13:21:48Zcom_2072_1057col_2072_478916col_2072_478917col_2072_478927

Cytocompatibility and Microbiological Effects of Ti6Al4V Particles Generated During Implantoplasty on Human Fibroblasts, Osteoblasts, and Multispecies Oral Biofilm Vegas Bustamante, Erika Toledano Serrabona, Jorge Sánchez Garcés, Ma. Ángeles Barbosa de Figueiredo, Rui Pedro Demiquels, Elena Gil, J. Delgado, Luís María Sanmartí Garcia, Gemma Camps Font, Octavi Implants dentals Fibroblasts Biofilms Dental implants Fibroblasts Biofilms Objectives: This study aimed to evaluate the cytotoxic effects of Ti6Al4V particles and implantoplasty (IP)-treated surfaces on human fibroblasts and osteoblasts, and to investigate the influence of these particles on multispecies oral biofilm formation. Methods: Ti6Al4V particles generated during implantoplasty were collected. Human fibroblasts (HFF-1) and osteoblast-like cells (SaOs-2) were used to assess cytotoxicity through indirect lactate dehydrogenase (LDH) assays. Multispecies biofilms composed of Streptococcus oralis, Actinomyces viscosus, Veillonella parvula and Porphyromonas gingivalis were evaluated based on colony-forming units (CFUs) and metabolic activity. Fibroblasts and osteoblasts were co-cultured with biofilm-contaminated particles for 2, 4 and 6 h. Cell morphology and biofilm association were examined by phase-contrast microscopy, while metabolic activity was measured spectrophotometrically. Results: IP-treated surfaces showed no significant cytotoxicity (metabolic activity > 92%, LDH < 20%). Ti6Al4V particles selectively promoted A. viscosus and V. parvula growth (metabolic activity increases of ≈192% and ≈203%; CFU significantly higher versus controls, p < 0.05). Co-culture with biofilm-contaminated particles drastically reduced cell activity (fibroblasts < 25%, osteoblasts < 10%), whereas bacteria-free particles did not. Conclusions: Biofilm-contaminated particles released during implantoplasty markedly impair fibroblast and osteoblast cytocompatibility and selectively alter bacterial growth, whereas IP-treated surfaces per se are biocompatible. Minimizing particle dissemination and bacterial contamination during IP is therefore crucial. 2026-03-12T18:32:14Z 2026-03-12T18:32:14Z 2025-12-15 2026-03-12T18:32:20Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 1996-1944 https://hdl.handle.net/2445/228060 766580 41470397 eng Reproducció del document publicat a: https://doi.org/10.3390/ma18245626 Materials, 2025, vol. 18, num.24 https://doi.org/10.3390/ma18245626 cc-by (c) Vegas-Bustamante, E. et al., 2025 http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess 18 p. application/pdf MDPI Articles publicats en revistes (Odontoestomatologia)