2026-04-13T16:53:33Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/2260102026-04-07T21:52:49Zcom_2072_1057col_2072_478781col_2072_478917

00925njm 22002777a 4500 dc Rodríguez Concepción, Manuel author Ahumada, Iván author Diez Juez, Ester author Sauret-Güeto, Susanna author Lois Rojas, Luisa María author Gallego, Francisco author Carretero Paulet, Lorenzo author Campos Martínez, Narciso author Boronat i Margosa, Albert author 2026-01-23T10:26:43Z 2026-01-23T10:26:43Z 2001 2026-01-23T10:26:43Z The recently discovered 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for the biosynthesis of plastid isoprenoids (including carotenoids) is not fully elucidated yet despite its central importance for plant life. It is known, however, that the first reaction completely specific to the pathway is the conversion of 1-deoxy-D-xylulose 5-phosphate (DXP) into MEP by the enzyme DXP reductoisomerase (DXR). We have identified a tomato cDNA encoding a protein with homology to DXR and in vivo activity, and show that the levels of the corresponding DXR mRNA and encoded protein in fruit tissues are similar before and during the massive accumulation of carotenoids characteristic of fruit ripening. The results are consistent with a non-limiting role of DXR, and support previous work proposing DXP synthase (DXS) as the first regulatory enzyme for plastid isoprenoid biosynthesis in tomato fruit. Inhibition of DXR activity by fosmidomycin showed that plastid isoprenoid biosynthesis is required for tomato fruit carotenogenesis but not for other ripening processes. In addition, dormancy was reduced in seeds from fosmidomycin-treated fruit but not in seeds from the tomato yellow ripe mutant (defective in phytoene synthase-1, PSY1), suggesting that the isoform PSY2 might channel the production of carotenoids for abscisic acid biosynthesis. Furthermore, the complete arrest of tomato seedling development using fosmidomycin confirms a key role of the MEP pathway in plant development. Biologia molecular vegetal Tomàquets Biotecnologia vegetal Plant molecular biology Tomatoes Plant biotechnology 1-Deoxy-D-xylulose 5-phosphate reductoisomerase and plastid isoprenoid biosynthesis during tomato fruit ripening